Expression, purification, and characterization of recombinant NS-1, the porcine parvovirus non-structural protein

文献类型: 外文期刊

第一作者: Qi, Ting

作者: Qi, Ting;Cui, Shang-jin

作者机构:

关键词: Antibodies;Fusion protein;Vaccination;Antibodies;Fusion protein;Vaccination;Vaccines;Western blotting;protein purification;Escherichia coli;Porcine parvovirus

期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:2.014; 五年影响因子:2.001 )

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收录情况: SCI

摘要: The non-structural protein NS-1 of porcine parvovirus (PPV) could be a useful antigen for differentiating pigs infected with PPV from those vaccinated with inactivated whole virus. NS-1 was expressed in Escherichia coli using the pET-32a (+) vector. The fusion protein, which was expressed at a high level, was similar antigenically to the native NS-1 protein as determined by Western blot assay using polyclonal antibodies from pigs vaccinated with alive PPV vaccine. A simple procedure was used to purify the fusion protein. This research lays the foundation for using the NS-1 protein for clinical diagnosis of pigs infected with PPV vs. those vaccinated with inactivated whole virus vaccine.

分类号: R737`R37

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