Analysis of Resistance to Powdery Mildew in Wheat Based on Expressed Sequence Tags (EST) Technique

文献类型： 外文期刊

第一作者： Luo, M

作者： Luo, M;Kong, XY;Jiang, T;Jia, C;Zhou, RH;Jia, JZ

作者机构：

关键词： wheat;erysiphe graminis;expressed sequence tags;disease resistance gene

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

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年卷期：

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收录情况： SCI

摘要： Powdery mildew is a serious disease of wheat in China. As part of ITEC (International Triteace EST cooperation), EST (expressed sequence tags) technique was used to explore the gene expression in leaf induced by Erysiphe graminis DC. A conventional cDNA library was constructed, and a total of 1 500 clones picked randomly from the library were sequenced, three hundred and eighty-seven ESTs of them were unique, which got the Accession Number in GenBank. About 49.4% ESTs showed significant similarity to functions of known sequences in GenBank. There are 196 ESTs' with functions not able to be determined, and eighty-four ESTs were demonstrated to be novel sequences. High-density dot membranes from unique clones were produced, and several disease resistance related genes were screened by differential hybridization.

分类号： Q94

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