A primary sodium pump gene of the moderate halophile Halobacillus dabanensis exhibits secondary antiporter properties

文献类型： 外文期刊

第一作者： Yang, LF

作者： Yang, LF;Jiang, JQ;Zhang, B;Zhao, BS;Wang, L;Yang, SS

作者机构：

关键词： moderate halophile;Halobacillus dabanensis;primary sodium pump;Na+/H+ antiporter;NADH-QUINONE OXIDOREDUCTASE;NA+-TRANSLOCATING NADH;ESCHERICHIA-COLI;COMPLEX-I;KLEBSIELLA-PNEUMONIAE;VIBRIO-ALGINOLYTICUS;MARINE BACTERIUM;NDH-I;TRANSPORTERS;CIRCULATION

期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：3.575； 五年影响因子：3.381 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The primary sodium pump has been proved to be involved in Na+ extrusion of bacteria. In our present study, a novel gene encoding a putative primary sodium pump was cloned from chromosomal DNA of moderate halophile Halobacillus dabanensis D-8 by functional complementation, which expression resulted in the growth of antiporter-deficient Escherichia coli strain KNabc in the presence of 0.2 M NaCl. The gene was sequenced and designated nap. The deduced amino acid sequence of Nap has 56% identity to NADH dehydrogenase of Bacillus cereus and 55% to NADH oxidase of Bacillus halodurans C-125. E coli KNabc carrying nap exhibited resistance to uncoupler CCCP (carbonyl-cyanide m-chlorophenylhydrazone). Everted membrane vesicles prepared from E. coli KNabc carrying nap exhibited secondary Na+/H+ antiporter activity, and nap also supported the growth of respiratory-deficient E coli ANN0222 lacking NADH dehydrogenase. Based on these results, we proposed that Nap possessed both characteristics of secondary Na+/H+ antiporter and primary sodium pump. (c) 2006 Elsevier Inc. All rights reserved.

分类号： Q5

  • 相关文献

[1]A Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus dabanensis D-8(T): cloning and molecular characterization. Yang, LF,Jiang, JQ,Zhao, BS,Zhang, B,Feng, DQ,Lu, WD,Wang, L,Yang, SS.

[2]Reclassification of Bacillus saliphilus as Alkalicoccus saliphilus gen. nov., comb. nov., and description of Alkalicoccus halolimnae sp nov., a moderately halophilic bacterium isolated from a salt lake. Zhao, Baisuo,Zhang, Shanshan,Liu, Kang,Yan, Yanchun,Zhao, Baisuo,Li, Jun,Lu, Weidong,Liu, Kang.

[3]Isolation and Polyphasic Investigation of the Carotenoids-producing Marine Bacterium from Trachurus murphyi Chile Fishery. Zhang, Xiaoling,Yang, Qiao,Zhang, Heng,Huang, Hongliang. 2013

[4]Effects of postprandial starvation on mRNA expression of endocrine-, amino acid and peptide transporter-, and metabolic enzyme-related genes in zebrafish (Danio rerio). Tian, Juan,He, Gen,Mai, Kangsen,Liu, Chengdong,Tian, Juan.

[5]Expression of sucrose metabolism and transport genes in cassava petiole abscission zones in response to water stress. Liao, W. B.,Li, Y. Y.,Lu, C.,Peng, M.. 2017

[6]Affinity purification of metalloprotease from marine bacterium using immobilized metal affinity chromatography. Li, Shangyong,Wang, Linna,Yang, Juan,Bao, Jing,Liu, Junzhong,Hao, Jianhua,Sun, Mi,Li, Shangyong,Wang, Linna,Yang, Juan,Bao, Jing,Liu, Junzhong,Hao, Jianhua,Sun, Mi,Li, Shangyong,Wang, Linna,Yang, Juan,Bao, Jing,Liu, Junzhong,Hao, Jianhua,Sun, Mi,Lin, Shengxiang.

[7]Overexpression of a putative maize calcineurin B-like protein in Arabidopsis confers salt tolerance. Wang, Maoyan,Gu, Dan,Liu, Tingsong,Wang, Zhaoqiang,Guo, Xiying,Hou, Wei,Bai, Yunfeng,Chen, Xiaoping,Wang, Guoying.

[8]Cloning and expression analysis of a vacuolar Na+/H+ antiporter gene from Alfalfa. Yang, QC,Wu, MS,Wang, PQ,Kang, JM,Zhou, XL.

[9]The wheat NHX antiporter gene TaNHX2 confers salt tolerance in transgenic alfalfa by increasing the retention capacity of intracellular potassium. Zhang, Yan-Min,Zhang, Hong-Mei,Liu, Zi-Hui,Li, Hui-Cong,Guo, Xiu-Lin,Li, Guo-ang.

[10]Cloning and characterization of a Na+/H+ antiporter gene of the moderately halophilic bacterium Halobacillus aidingensis AD-6(T). Zou, Ya Jie,Yang, Li Fu,Wang, Lei,Yang, Su Sheng,Zou, Ya Jie,Yang, Li Fu,Wang, Lei,Yang, Su Sheng,Yang, Li Fu,Yang, Li Fu. 2008

[11]Molecular characterization of GmNHX2, a Na+/H+ antiporter gene homolog from soybean, and its heterologous expression to improve salt tolerance in Arabidopsis. Zhou GuoAn,Guan RongXia,Li YingHui,Chang RuZhen,Qiu LiJuan. 2009

[12]Metagenomic cloning and characterization of Na+/H+ antiporter genes taken from sediments in Chaerhan Salt Lake in China. Wang, Xiaoyu,Chen, Sanfeng,Xu, Feng.

[13]Zinc improves salt tolerance by increasing reactive oxygen species scavenging and reducing Na+ accumulation in wheat seedlings. Xu, L. H.,Wang, W. Y.,Xu, J.,Xu, L. H.,Guo, J. J.,Shi, D. Q.,Xu, J.,Qin, J.,Shi, D. Q.,Li, Y. L..

[14]Renaturation and purification of ApxII toxin of Actinobacillus pleuropneumoniae. Wang, Chunlai,Liu, Siguo,Peng, Yonggang,Shao, Meili,Wang, Yong,Gong, Qiang,Chang, Yuehong,Liu, Jiandong,Liu, Huifang,Liu, Di,Kong, Xiangang.

[15]Co-ordinate expression of glycine betaine synthesis genes linked by the FMDV 2A region in a single open reading frame in Pichia pastoris. Wang, Sanhong,Yao, Quanhong,Tao, Jianmin,Qiao, Yushan,Zhang, Zhen.

[16]Molecular and biochemical characterization of a distinct tyrosinase involved in melanin production from Aeromonas media. Wan, Xia,Chai, Baozhong,Liao, Yi,Ye, Tao,Shen, Ping,Chen, Xiangdong,Wan, Xia,Su, Ying.

[17]Isolation and characterization of induced genes under drought stress at the flowering stage in maize (Zea mays). Li, Hui-Yong,Wang, Tian-Yu,Shi, Yun-Su,Fu, Jun-Jie,Song, Yan-Chun,Wang, Guo-Ying,Li, Yu.

[18]Codon optimization of Bacillus licheniformis beta-1,3-1,4-glucanase gene and its expression in Pichia pastoris. Teng, Da,Fan, Ying,Yang, Ya-lin,Tian, Zi-gang,Luo, Jin,Wang, Jian-hua.

[19]Purification of green fluorescent protein using a two-intein system. Lu, Wei,Ping, Shuzhen,Zhang, Wei,Chen, Ming,Lin, Min,Zhao, Zhonglin,Dun, Baoqing,Jin, Dan,Xu, Ming-Qun.

[20]Expression of common carp growth hormone in the yeast Pichia pastoris and growth stimulation of juvenile tilapia (Oreochromis niloticus). Li, YH,Bai, JJ,Jian, Q,Ye, X,Lao, HH,Li, XH,Luo, JR,Liang, XF.

作者其他论文 更多>>

微信小程序