Transgenic cotton expressing synthesized scorpion insect toxin AaHIT gene confers enhanced resistance to cotton bollworm (Heliothis armigera) larvae
文献类型: 外文期刊
第一作者: Wu, Jiahe
作者: Wu, Jiahe;Luo, Xiaoli;Wang, Zhian;Liang, Aihua;Sun, Yi;Wu, Jiahe;Luo, Xiaoli;Wang, Zhian;Liang, Aihua;Sun, Yi;Wu, Jiahe;Tian, Yingchuan
作者机构:
关键词: Androctonus australis;bollworm control;cotton (transgenic);Gossypium hirsutum;scorpion toxin in cotton;PLANTS;INHIBITOR;PROTEIN;WEEVIL;VENOM
期刊名称:BIOTECHNOLOGY LETTERS ( 影响因子:2.461; 五年影响因子:2.457 )
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收录情况: SCI
摘要: A synthetic scorpion Hector Insect Toxin (AaHIT) gene, under the control of a CaMV35S promoter, was cloned into cotton via Agrobacterium tumefaciens-mediated transformation. Southern blot analyses indicated that integration of the transgene varied from one to more than three estimated copies per genome; seven homozygous transgenic lines with one copy of the T-DNA insert were then selected by PCR and Southern blot analysis. AaHIT expression was from 0.02 to 0.43% of total soluble protein determined by western blot. These homozygous transgenic lines killed larvae of cotton bollworm (Heliothis armigera) by 44-98%. The AaHIT gene could used therefore an alternative to Bt toxin and proteinase inhibitor genes for producing transgenic cotton crops with effective control of bollworm.
分类号: Q81
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