Wheat Cryptochromes: Subcellular Localization and Involvement in Photomorphogenesis and Osmotic Stress Responses

文献类型： 外文期刊

第一作者： Xu, Pei

作者： Xu, Pei;Xiang, Yang;Zhu, Huilan;Xu, Haibin;Zhang, Zhengzhi;Zhang, Caiqin;Zhang, Lixia;Ma, Zhengqiang;Xu, Pei;Xiang, Yang;Zhu, Huilan;Xu, Haibin;Zhang, Zhengzhi;Zhang, Caiqin;Zhang, Lixia;Ma, Zhengqiang;Xu, Pei

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期刊名称：PLANT PHYSIOLOGY （ 影响因子：8.34； 五年影响因子：8.972 ）

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收录情况： SCI

摘要： Cryptochromes (CRYs) are blue light receptors important for plant growth and development. Comprehensive information on monocot CRYs is currently only available for rice (Oryza sativa). We report here the molecular and functional characterization of two CRY genes, TaCRYla and TaCRY2, from the monocot wheat (Triticum aestivum),. The expression of TaCRYla was most abundant in seedling leaves and barely detected in roots and germinating embryos under normal growth conditions. The expression of TaCRYl in germinating embryos was equivalent to that in leaves and much higher than the TaCRYla counterpart. Transition from dark to light slightly affected the expression of TaCRYla and TaCRY2 in leaves, and red light produced a stronger induction of TaCRYla, Treatment of seedlings with high salt, polyethylene glycol, and abscisic acid (ABA) up-regulated TaCRYZ in roots and germinating embryos,, TaCRYla displays a light-responsive nucleocytoplasmic shuttling pattern similar to that of Arabidopsis (Arabidopsis thaliana) CRY1, contains nuclear localization domains in both the N and C termini, and includes information for nuclear export in its N-terminal domain. TaCRY2 was localized to the nucleus in the dark. Expression of TaCRYla-green fluorescent protein or TaCRYl-green fluorescent protein in Arabidopsis conferred a shorter hypocotyl phenotype under blue light. These transgenic Arabidopsis plants showed higher sensitivity to high-salt, osmotic stress, and ABA treatment during germination and postgermination development, and they displayed altered expression of stress/ABA-responsive genes. The primary root growth in transgenic seedlings was less tolerant of ABA. These observations indicate that TaCRYl and TaCRY2 might be involved in the ABA signaling pathway in addition to their role in primary blue light signal transduction.

分类号： Q945

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