Construction of AFLP-based genetic linkage maps for the Chinese shrimp Fenneropaeneus chinensis

文献类型： 外文期刊

第一作者： Tian Yi

作者： Tian Yi;Kong Jie;Wang WeiJi;Tian Yi;Tian Yi;Tian Yi

作者机构：

关键词： Molecular Genetics: Biochemistry and Molecular Biophysics

期刊名称：CHINESE SCIENCE BULLETIN （ 影响因子：1.649； 五年影响因子：1.738 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Fenneropaeneus chinensis is an important species in marine fishery resources and aquaculture in China. A genetic linkage map is essential for improving the efficiency of its breeding by marker-assisted selection and identifying commercially important genes. Linkage maps of F chinensis were constructed with an F2 mapping population (110 progenies) using amplified fragment length polymorphic (AFLP) marker in this study. Fifty-five AFLP primer combinations produced 532 AFLP markers fitting for map strategy in mapping family. The markers with 3:1 segregating ratios were analyzed using F2 intercross model for the common linkage map, while the markers with 1:1 ratio were analyzed using the pseudo-testcross strategy. The maps of male, female and common were constructed. The female map included 103 markers that formed 28 linkage groups, covering a total length of 1090 M All markers were linked with the linkage groups. Segregation distortion was observed for 6 of 103 markers in the female map. The average distance between markers was 14.53 cM and ranged from 4.4 to 24.8 M The male map included 144 markers that formed 35 linkage groups. Ten markers remained unlinked in male map. Segregation distortion was observed for 7 of 144 markers in the male map. The total distance of male map covered 1617 M The average distance between markers was 16.36 cM. The male map was 32.6% longer than the female map, which may reflect sex-specific recombination rates in Chinese shrimp. The common map was composed of 216 markers, including in 44 linkage groups covering a total distance of 1772.1 cM. Two markers remained unlinked. No distorted markers of 216 markers were shown in the common map. The distance between markers was 10.42 M An average estimated genome size for the Chinese shrimp was 2420 cM, which was consistent with the relative size of the Penaeid genome. The distribution of AFLP markers was relatively even in chromosomes of Chinese shrimp maps. The linkage analysis presented in this work provided some insight into the level of polymorphism and genetic variation of Chinese shrimp.

分类号： N1

  • 相关文献

[1]An IL-15 adjuvant enhances the efficacy of a combined DNA vaccine against Brucella by increasing the CD8(+) cytotoxic T cell response. Cai, Hong,Hu, Xi-Dan.

[2]A simple artificial microRNA vector based on ath-miR169d precursor from Arabidopsis. Liu, Chong,Zhang, Lan,Luo, Yanzhong,Fan, Yun-Liu,Wang, Lei,Liu, Chong,Sun, Jie,Wang, Ming-Bo.

[3]Development of SNP markers and haplotype analysis of the candidate gene for rhg1, which confers resistance to soybean cyst nematode in soybean. Li, Ying-Hui,Zhang, Chen,Ma, Zulu,Liu, Zhang-Xiong,Nan, Hai-Yang,Chang, Ru-Zhen,Qiu, Li-Juan,Gao, Zhong-Shan,Smulders, Marinus Johannes Maria,Ma, Zulu.

[4]Establishment and Characterization of the Fibroblast Line from Silkie Bantam. Li, L. F.,Guan, W. J.,Li, H.,Ma, Y. H..

[5]Transgene expression of enhanced green fluorescent protein in cloned rabbits generated from in vitro-transfected adult fibroblasts. Li, Shangang,Yin, Chunguang,Xu, Lingyang,Liu, Tao,Li, Hongbin,Du, Lixin,Guo, Yi,Shi, Jianjun,Xing, Fengying,Li, Yao,Chen, Xuejin,Zhang, Chuanshan.

[6]Generation and efficacy evaluation of a recombinant adenovirus expressing the E2 protein of classical swine fever virus. Sun, Yuan,Liu, Da-Fei,Wang, Yu-Fei,Liang, Bing-Bing,Cheng, Dan,Li, Na,Qi, Qiao-Fen,Zhu, Qing-Hu,Qiu, Hua-Ji.

[7]Evaluation of Echinococcus multilocularis tetraspanins as vaccine candidates against primary alveolar echinococcosis. Dang, Zhisheng,Kajino, Kiichi,Nakao, Ryo,Sugimoto, Chihiro,Dang, Zhisheng,Yagi, Kinpei,Kouguchi, Hirokazu,Oku, Yuzaburo,Matsumoto, Jun,Watanabe, Junichi,Matsumoto, Jun,Wakaguri, Hiroyuki,Toyoda, Atsushi,Toyoda, Atsushi.

[8]Characterization of the H5N1 Highly Pathogenic Avian Influenza Virus Derived from Wild Pikas in China. Zhou, Jiyong,Sun, Wenbo,Wang, Junhua,Guo, Junqing,Yin, Wei,Yan, Yan,Jiang, Xuetao,Zhou, Jiyong,Sun, Wenbo,Wang, Junhua,Guo, Junqing,Yin, Wei,Wu, Nanping,Li, Lanjuan,Yan, Yan,Jiang, Xuetao,Zhou, Jiyong,Sun, Wenbo,Wang, Junhua,Guo, Junqing,Yan, Yan,Chen, Hualan,Liao, Ming,Luo, Kaijian,Huang, Yu.

[9]Construction of Mammalian Cell Expression Vector for pAcGFP-bFLIP(L) Fusion Protein and Its Expression in Follicular Granulosa Cells. Yang, Run Jun,Li, Jun Ya,Zhang, Lu Pei,Gao, Xue,Chen, Jin Bao,Xu, Shang Zhong.

[10]The development and evaluation of a loop-mediated isothermal amplification (LAMP) method for detection of Babesia spp. infective to sheep and goats in China. Guan, Guiquan,Luo, Jianxun,Liu, Zhijie,Gao, Jinliang,Ma, Miling,Liu, Aihong,Dang, Zhisheng,Liu, Junlong,Ren, Qiaoyun,Jin, Yurong,Yin, Hong,Guan, Guiquan,Chauvin, Alain,Moreau, Emmanuelle,Inoue, Noboru,Thekisoe, Oriel M. M.,Sugimoto, Chihiro.

[11]Ri-mediated Transformation of Glycyrrhiza uralensis with a Squalene Synthase Gene (GuSQS1) for Production of Glycyrrhizin. Lu, Hong-Yu,Zhang, Hai-Chao,Gao, Shan-Lin,Liu, Jing-Mei,Yin, Tao.

[12]SSR based linkage and mapping analysis of C, a yellow cocoon gene in the silkworm, Bombyx mori. Zhao, Yun-Po,Li, Mu-Wang,Xu, An-Ying,Hou, Cheng-Xiang,Guo, Xi-Jie,Zhao, Yun-Po,Guo, Xi-Jie,Li, Ming-Hui,Guo, Qiu-Hong,Huang, Yong-Ping.

[13]Application of comparative genomics in chicken gene mapping. Tian, Yong,Lu, Lizhi,Wang, Deqian,Li, Guoqin,Yuan, Aiping,Yuan, Qingyan,Tian, Yong,Fu, Yan,Zhao, Jin.

[14]Cloning and characterization of a novel cysteine protease gene (HbCP1) from Hevea brasiliensis. Peng, Shi-Qing,Zhu, Jia-Hong,Li, Hui-Liang,Tian, Wei-Min.

[15]Identification and characterization of inosine 5-monophosphate dehydrogenase in Streptococcus suis type 2. Zhang, Xue-han,He, Kong-wang,Duan, Zhi-tao,Zhou, Jun-ming,Yu, Zheng-yu,Ni, Yan-xiu,Zhang, Xue-han,He, Kong-wang,Duan, Zhi-tao,Zhou, Jun-ming,Yu, Zheng-yu,Ni, Yan-xiu,Zhang, Xue-han,He, Kong-wang,Duan, Zhi-tao,Zhou, Jun-ming,Yu, Zheng-yu,Ni, Yan-xiu,Lu, Cheng-ping.

[16]'Candidatus Phytoplasma tamaricis', a novel taxon discovered in witches'-broom-diseased salt cedar (Tamarix chinensis Lour.). Zhao, Yan,Sun, Qingrong,Wei, Wei,Davis, Robert E.,Wu, Wei,Liu, Qingzhong,Sun, Qingrong,Liu, Qingzhong.

[17]OsNOA1/RIF1 is a functional homolog of AtNOA1/RIF1: implication for a highly conserved plant cGTPase essential for chloroplast function. Liu, Hongjia,Li, Sujuan,Huang, Guo,Guo, Peng,Tao, Yuezhi,Liu, Hongjia,Chu, Hung,Lo, Clive,Lau, Edward,Lam, Maggie P. Y.,Chu, Ivan K.,Wang, Junqi,Jiang, Liwen.

[18]The Arabidopsis Chaperone J3 Regulates the Plasma Membrane H+-ATPase through Interaction with the PKS5 Kinase. Yang, Yongqing,Xie, Changgen,Zhao, Jinfeng,Guo, Yan,Yang, Yongqing,Xie, Changgen,Deng, Xing Wang,Yang, Yongqing,Guo, Yan,Qin, Yunxia,Liu, Dafa,Zhao, Feiyi,Chen, Shouyi,Fuglsang, Anja T.,Palmgren, Michael G.,Schumaker, Karen S..

[19]Molecular mapping of powdery mildew resistance gene PmHNK in winter wheat (Triticum aestivum L.) cultivar Zhoumai 22. Xu, W. G.,Li, C. X.,Hu, L.,Zhang, L.,Zhang, J. Z.,Dong, H. B.,Wang, G. S.,Li, C. X..

[20]Cloning and Expression of the Porcine MEF2a Gene. Zhang, Dong-jie,Liu, Di,Yang, Guo-wei,Gao, Zhi-sheng.

作者其他论文 更多>>

微信小程序