Event-specific Qualitative And Quantitative Pcr Detection Of Genetically Modified Rapeseed Topas 19/2

文献类型： 外文期刊

第一作者： Wu, Gang

作者： Wu, Gang;Wu, Yuhua;Xiao, Ling;Lu, Changming

作者机构：

关键词： topas 19/2;genetically modified organism;herbicide-tolerant rapeseed;event-specific method;real-time pcr;quantitative pcr

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The herbicide-tolerant transgenic rapeseed Topas 19/2 (synonym HCN92) has been approved for environmental release in Canada, Japan, Australia and the USA, and exported to a number of other countries as raw material. The purpose of this study was to establish event-specific qualitative and quantitative detection methods for Topas 19/2. The 3'-integration junction sequence spanning the host plant DNA and the integrated transgene of the Topas 19/2 event was isolated and identified. The event-specific qualitative detection method was established to produce an amplicon of 110 basepairs (bp) with an absolute detection limit of 10 initial template copies. The event-specific quantitative detection method was developed with the limit of detection (LOD) and limit of quantification (LOQ) being approximately 5 and 50 initial template copies, respectively. The developed real-time PCR systems were assessed using two mixed rape-seed samples with known Topas 19/2 contents. Expected results were obtained.

分类号： TS201`TS264`TS201.2

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