Acetolactate Synthase Gene Pro line (197) Mutations Confer Tribenuron-Methyl Resistance in Flixweed (Descurainia sophia) Populations from China

文献类型: 外文期刊

第一作者: Zhang, Chao Xian

作者: Zhang, Chao Xian;Wei, Shou Hui;Li, Xiang Ju;Cui, Hai Lan;Zhang, Chao Xian;Wei, Shou Hui;Li, Xiang Ju;Zhang, Hong Jun;Zhang, Yan Qiu;Wang, Gui Qi

作者机构:

关键词: tribenuron-methyl: 101200-48-0;pesticide;herbicide;acetolactate synthase: 9027-45-6;EC 2.2.1.6;serine;proline 197;leucine;herbicide resistance

期刊名称:WEED SCIENCE ( 影响因子:2.713; 五年影响因子:2.868 )

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收录情况: SCI

摘要: The molecular basis of resistance to tribenuron-methyl, an acetolactate synthase (ALS)-inhibiting herbicide was investigated in four resistant (R) and three susceptible (S) flixweed populations. The resistance level in the R populations was assessed in whole-plant pot experiments in a greenhouse, and resistance indices ranged from 723 to 1422. The ALS genes of the three S populations and four R populations were cloned and sequenced, and the full coding sequence of the ALS gene of flixweed was 2,004 bp. The sequences of the ALS genes of the three S populations collected from Shaanxi, Gansu, and Tianjin were identical. Comparison of the ALS gene sequences of the S and R populations with Arabidopsis revealed that proline at position 197 of the ALS gene was substituted by leucine in R population SSX-2, by alanine in R population SSX-3, and by serine in R populations TJ-2 and GS-2. In another study of two R flixweed populations from Hebei and Shaanxi, resistance was also related to mutation at position 197 of the ALS gene. Both studies confirmed tribenuron-methyl resistance in flixweed in China, with the resistance mechanism being conferred by specific ALS point mutations at amino acid position 197.

分类号: S451

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