Evaluation of genetic identity and variation in cultivars of Pyrus pyrifolia (Burm.f.) Nakai from China using microsatellite markers

文献类型： 外文期刊

第一作者： Cao, Y.

作者： Cao, Y.;Yang, J.;Zhang, S.;Cao, Y.;Tian, L.;Gao, Y.

作者机构：

关键词： microsatellite marker;allele;simple sequence repeatZhejiang: China;Asia;Palearctic region;Yunnan: China;Asia;Palearctic region;Jiangxi: China;Asia;Palearctic region;Jiangxi Province: China;Asia;Palearctic region;Nakai: China;Asia;Palearctic region;Jiangwantangli province: China;Asia;Palearctic region;heterozygosity;genetic diversity;genetic evolution;genetic identity;polymorphic information;gen bank accession data

期刊名称：JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY （ 影响因子：1.641； 五年影响因子：1.616 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： One-hundred and six accessions of Pyrus, including 87 local Chinese accessions of P pyrifolia, were identified genetically using nine microsatellite (simple sequence repeat) loci developed from apple and pear. A total of 147 putative alleles were obtained. Among the :106 accessions, 100 could be differentiated successfully, except for three pairs of synonymous accessions or mutants. For the 87 accessions of Chinese P pyrifolia, the number of putative alleles per locus ranged from ten to 21, with an average of 15.3. The observed heterozygosity and polymorphic information content were 0.60 and 0.75, respectively. A phenogram based on the SSR genotypes was obtained. The 87 accessions of Chinese P pyrifolia clustered into seven groups, based on their geographical origin. Most P bretschneideri cultivars grouped together with the P pyrifolia accessions from the same geographical areas. Some Japanese pear cultivars (i.e., 'Choujuurou','Housui','Niitaka', 'Okusankichi', and 'Shinseiki) grouped together with some accessions of P pyrifolia from the Yunnan, Jiangxi, and Zhejiang Provinces of China. The similarity coefficient between 'Jiangwantangli' from Jiangxi Province and a Japanese pear cultivar, 'Choujuurou', was 0.80. Cultivars of R communis and P ussuriensis showed distant relationships with those of P. pyrifolia.

分类号： S6

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