Comparative genomic analysis of five pairs of virulent parental/attenuated vaccine strains of PRRSV

文献类型： 外文期刊

第一作者： Zhou, Yan-Jun

作者： Zhou, Yan-Jun;Jiang, Yi-Feng;Tong, Guang-Zhi;An, Tong-Qing;Tian, Zhi-Jun;Xiao, Yan;Peng, Jin-Mei;Chen, Jin;Hao, Xiao-Fang;Tong, Guang-Zhi

作者机构：

关键词： gene: mutation;E protein;NSP6 protein;GP5 protein;envelope-associated structural protein;GP2 protein;NSP8 protein;PRRSV attenuated vaccine: immunologic-drug;immunostimulant-drug;vaccine;parenteral administration;attenuation process

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Porcine reproductive and respiratory syndrome virus (PRRSV), the causative agent of porcine reproductive and respiratory syndrome, is responsible for serious disease in pigs resulting in substantial economic losses in the porcine industry. An attenuated vaccine strain, HuN4-F112, was obtained by passaging virulent PRRSV strain HuN4 on Marc-145 cells (for 112 passages), and the full-genomic sequence was determined. To understand the molecular basis of attenuation of PRRSV, we compared and analyzed the genomic sequences of HuN4/HuN4-F112, together with those of other four virulent parental/attenuated vaccine strains. Among the 19 PRRSV proteins, two (NSP6 and NSP8) were highly conserved, without any mutations and considered irrelative to attenuation. The mutation rates of envelope-associated structural proteins were obviously higher than those of most non-structural proteins. It is interesting that the gene of the smallest structural protein, E protein, had the highest mutation rate among all of the structural genes analyzed, and also harbored a highly variable region. Our results indicate that determinants of PRRSV attenuation are multigenic products of both non-structural and structural genes. To our knowledge, this is the first report showing that the envelope-associated structural proteins (including E and GP2-GP5 proteins) may play a significant role. These findings contribute towards our understanding of PRRSV attenuation and will provide an important clue for further study

分类号： S8

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