Effects of 1-MCP and ethylene on expression of three CAD genes and lignification in stems of harvested Tsai Tai (Brassica chinensis)
文献类型: 外文期刊
第一作者: Zhang, Lubin
作者: Zhang, Lubin;Wang, Guang;Liu, Jianshuang;Cai, Jianghua;Rao, Xiuwen;Zhang, Lijuan;Zhong, Juanjuan;Zhu, Shijiang;Zhang, Lubin;Chang, Jinmei;Xie, Jianghui
作者机构:
关键词: cinnamyl alcohol dehydrogenase;ethylene;1-MCP;senescence;lignification;brassica chinensis
期刊名称:FOOD CHEMISTRY ( 影响因子:7.514; 五年影响因子:7.516 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: Tsai Tai (Brassica chinensis) is produced mainly in China but also consumed overseas. The stems of Tsai Tai are subject to toughening due to lignification. Cinnamyl alcohol dehydrogenase (CAD) is a key enzyme involved in lignin biosynthesis. Three full-length CAD cDNAs were isolated, encoding proteins of 288, 323, and 323 amino acids, respectively. Sequence analysis showed that they share a highly conserved putative NAD/NADP(H)-binding site at the amino terminus. All the three BcCAD genes responded to 1-MCP and ethylene within 2 h. Ethylene up-regulated expression of BcCADl-1 and BcCAD2, while 1-MCP down-regulated them. Ethylene increased yellow leaves and stem lignin, and 1-MCP decreased them. These results suggest ethylene is involved in lignin growth in Tsai Tai, and induced expression of BcCAD1-l and BcCAD2 could contribute to lignification. They also suggest removal of ethylene and application of 1 -MCP could extend shelf-life and knockout of certain CAD genes could produce lines that store well.
分类号: NULL
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