Development of a Lateral Flow Colloidal Gold Immunoassay Strip for the Rapid Detection of Olaquindox Residues
文献类型: 外文期刊
第一作者: Song, Chunmei
作者: Song, Chunmei;Liu, Qingtang;Zhi, Aimin;Yang, Jifei;Zhi, Yubao;Li, Qingmei;Hu, Xiaofei;Deng, Ruiguang;Zhang, Gaiping;Song, Chunmei;Casas, Justin;Tang, Liang
作者机构:
关键词: olaquindox;test strip;colloidal gold;immunoassay;rapid test
期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:5.279; 五年影响因子:5.269 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: A rapid immunochromatographic lateral flow test strip of competitive format has been developed for the specific determination of olaquindox (OLA) residues in pig urine and muscle tissues. The sensitivity of the test strip was found to be 1.58 ± 0.27 μg/kg and 1.70 ± 0.26 μg/kg of OLA in pig urine and muscle tissues, and the lower detection limit was 0.27 ± 0.08 μg/kg and 0.31 ± 0.07 μg/kg respectively. For negative pig urine and muscle samples spiked with 4, 12, and 36 μg/kg, the recovery range was 83.0-94.0% and 78.8-87.4% and the coefficient of variation scope [CV (%)] was 3.17-7.41% and 4.66-7.6496 respectively. Parallel analysis of OLA samples from pig urine and muscle tissue showed comparable results from the test strip and HPLC. Each test requires 5-8 min, and the test strip can provide a useful screening method for quantitative, semiquantitative, or qualitative detection of OLA residues.
分类号: R15
- 相关文献
作者其他论文 更多>>
-
Development of an Enzyme-Linked Immunosorbent Assay Based on a Monoclonal Antibody for the Rapid Detection of Citrinin in Wine
作者:Yang, Xingdong;Qu, Yang;Wang, Chenchen;Wu, Lihua;Yang, Xingdong;Hu, Xiaofei;Yang, Xingdong;Hu, Xiaofei
关键词:citrinin; monoclonal antibody; ic-ELISA; wine
-
Palmitoylation enhances the stability of porcine epidemic diarrhea virus spike protein by antagonizing its degradation via chaperone-mediated autophagy to facilitate viral proliferation
作者:Qian, Qisheng;Zhang, Gaiping;Qian, Qisheng;Zhao, Shuang-shuang;Yang, Lei;Xing, Guangxu;Li, Rui;Qiao, Songlin;Zhang, Gaiping;Qian, Qisheng;Zhang, Gaiping;Chen, Yumei;Liang, Chao;Wang, Haili;Wang, Aiping
关键词:porcine epidemic diarrhea virus; spike protein; palmitoylation; protein stability; chaperone-mediated autophagy
-
Fine mapping of conserved neutralizing epitopes within the VP2 protein of Senecavirus A using monoclonal antibodies
作者:Zou, Wanying;Li, Chunzhen;Meng, Zekun;Zhang, Gaiping;Zou, Wanying;Li, Qingmei;Li, Chunzhen;Meng, Zekun;Sun, Yaning;Yang, Suzhen;Guo, Junqing;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping;Zhang, Gaiping
关键词:Senecavirus A; VP2 protein; Monoclonal antibodies; Neutralizing epitope
-
Identification of linear B-cell epitopes of Senecavirus A VP2 protein using monoclonal antibodies
作者:Jiang, Yao;Zhang, Gaiping;Guo, Zhenhua;Weng, Maoyang;Chen, Linlin;Li, Qingmei;Qiao, Songlin;Zhang, Gaiping;Zhang, Lei;Zhang, Gaiping;Zhang, Gaiping
关键词:Senecavirus A; VP2 protein; monoclonal antibodies; B-cell epitopes; spatial structure
-
Detection method for identifying duck hepatitis A virus 3 virulent and attenuated strains based on RPA CRISPR single-base recognition system
作者:Chen, Lei;Zhang, Qiaoli;Sun, Wenbo;Mauk, Michael G.;Li, Qingmei
关键词:DHAV-3 virulent and attenuated strains; RPA-CRISPR; Single-base recognition
-
An indirect competitive ELISA for determination of guanidine acetic acid in animal feed
作者:Yang, Xingdong;Li, Lili;Qu, Yang;Wu, Lihua;Wang, Chenchen;He, Beibei;Zang, Zhenchun;Yang, Xingdong;Hu, Xiaofei
关键词:Guanidino acetic acid; Immunogen; Monoclonal antibody; ELISA; Animal feed
-
PEDV Nsp14 induces mitophagy-mediated degradation of MAVS to antagonize host innate immunity and facilitate viral proliferation
作者:Yang, Lei;Zhang, Gaiping;Yang, Lei;Qian, Qisheng;Chen, Xin-xin;Xing, Guangxu;Qiao, Songlin;Li, Rui;Zhang, Gaiping;Yang, Lei;Zhang, Gaiping;Sun, Yan-gang;Zhang, Jia-qing;Xing, Bao-song
关键词:PEDV; Nsp14; mitophagy; IFN-beta; NDP52