Molecular analysis shows differential expression of R-spondin1 in zebrafish (Danio rerio) gonads

文献类型： 外文期刊

第一作者： Zhang, Yanmei

作者： Zhang, Yanmei;Li, Fei;Liu, Jiangdong;Liu, Na;Yu, Qixing;Sun, Dongchang

作者机构：

关键词： sex determination;in situ hybridization.;R-spondin1;Differential gene expression;Internet resource.

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： R-spondin1 (RSPO1) is a potential female-determining gene in human (Homo sapiens) and mouse (Mus musculus). Its differential expression in these mammals is correlated with signaling for sex determination. As a way of studying sex determination in fish we cloned and analyzed a RSPO1 gene in zebrafish (Danio rerio). Using real-time PCR, we observed that RSPO1 is expressed more strongly in ovaries than in testes, suggesting that RSPO1 may have a role in gonad differentiation. High RSPO1 expression was detected in some non-gonadal organs like muscle and kidneys. In situ hybridization results demonstrate that RSPO1 is expressed in premature germ cells, in oogonia and primary oocytes in ovaries and in spermatogonia and spermatocytes in testes. It is also expressed in gonad somatic cells during gonadal development: in granulosa cells and theca cells of early and late cortical-alveolar stage follicles in ovaries, and in Leydig cells in testes. This differential expression may indicate that RSPO1 has a role(s) in zebrafish gonad development and differentiation. By fusing zebrafish RSPO1 with a green fluorescent protein gene, we found that RSPO1 is located in the cytosol and Golgi apparatus but not the nucleus of fish epithelioma papulosum cyprinid (EPC) cells. These preliminary findings suggest some aspects of RSPO1 like differential expression linked to sex determination may be conserved in fish while other aspects like subcellular localization differ from the mammalian RSPO1.

分类号： Q7

  • 相关文献

[1]Construction of Two BAC Libraries from Half-Smooth Tongue Sole Cynoglossus semilaevis and Identification of Clones Containing Candidate Sex-Determination Genes. Shao, Chang-Wei,Chen, Song-Lin,Xu, Jian-Yong,Sha, Zhen-Xia,Dong, Xiao-Li,Shao, Chang-Wei,Scheuring, Chantel F.,Zhang, Hong-Bin,Shao, Chang-Wei,Scheuring, Chantel F.,Zhang, Hong-Bin.

[2]Characterization and expression pattern of r-spondin1 in Cynoglossus semilaevis. Li, Hailong,Xu, Wenteng,Zhu, Ying,Zhang, Ning,Ma, Jialu,Sun, Ai,Cui, Zhongkai,Gao, Fengtao,Wang, Na,Shao, Changwei,Dong, Zhongdian,Li, Yangzhen,Li, Hailong,Xu, Wenteng,Zhu, Ying,Zhang, Ning,Ma, Jialu,Cui, Zhongkai,Gao, Fengtao,Wang, Na,Shao, Changwei,Dong, Zhongdian,Li, Yangzhen,Sun, Ai. 2017

[3]Relationship Between Gene Differential Expression of Leaves in Full Opening Flower Stages of Hybrids & Their Parents and Heterosis in Pest-resistant Cotton. XING Chao-Zhu,ZHAO Yun-Lei,YU Shu-Xun,ZHANG Xian-Long,GUO Li-Ping,WANG Hai-Lin. 2006

[4]Comparison of gene expression patterns between porcine cumulus-oocyte complexes and naked oocytes. Zhu, G. Y.,Feng, S. T.,Li, J. T.,Mu, Y. L.,Pan, D. K.,Guo, B. R.. 2007

[5]Strand-specific RNA-Seq transcriptome analysis of genotypes with and without low-phosphorus tolerance provides novel insights into phosphorus-use efficiency in maize. Du, Qingguo,Wang, Kai,Xu, Cheng,Zou, Cheng,Xie, Chuanxiao,Xu, Yunbi,Li, Wen-Xue. 2016

[6]Estimating Plasmodiophora brassicae gene expression in lines of B. rapa by RT-PCR. Wu, Li-yan,Li, Shi-kai,Gong, Ya-ju,Zhong, Li,He, Jiang-Ming,Siemens, Johannes,Ludwig-Mueller, Jutta. 2012

[7]Comparative Transcriptome Analysis Revealed Genes Commonly Responsive to Varied Nitrate Stress in Leaves of Tibetan Hulless Barley. Zeng, Xingquan,Wang, Yulin,Xu, Qijun,Yuan, Hongjun,Tang, Yawei,Nyima, Tashi,Wei, Zexiu,Zeng, Xingquan,Wang, Yulin,Xu, Qijun,Yuan, Hongjun,Tang, Yawei,Nyima, Tashi,Wei, Zexiu,Zeng, Xingquan,Wang, Yulin,Xu, Qijun,Yuan, Hongjun,Tang, Yawei,Qin, Cheng,Bai, Lijun. 2016

[8]Molecular characterization and expression analysis of the porcine caveolin-3 gene. Zhu, Zhengmao,Li, Yong,Mo, Delin,Li, Kui,Zhao, Shuhong.

[9]Molecular cloning and characterization of cotton cDNAs expressed in developing fiber cells. Guang-Rong ZHAO,Jin-Yuan LIU,Xiong-Ming DU. 2001

[10]Transcriptome analysis reveals the effect of pre-harvest CPPU treatment on the volatile compounds emitted by kiwifruit stored at room temperature. Luo, Jing,Guo, Linlin,Huang, Yunan,Wang, Chao,Qiao, Chengkui,Pang, Rongli,Li, Jun,Pang, Tao,Wang, Ruiping,Xie, Hanzhong,Fang, Jinbao. 2017

[11]Cytoplasmic polyhedrosis virus-induced differential gene expression in two silkworm strains of different susceptibility. Gao, Kun,Deng, Xiang-yuan,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie.

[12]Identification of differential gene expression profiles between winter dormant and sprouting axillary buds in tea plant (Camellia sinensis) by suppression subtractive hybridization. Wang, Xinchao,Hao, Xinyuan,Ma, Chunlei,Cao, Hongli,Yue, Chuan,Wang, Lu,Zeng, Jianming,Yang, Yajun,Wang, Xinchao,Hao, Xinyuan,Ma, Chunlei,Cao, Hongli,Yue, Chuan,Wang, Lu,Zeng, Jianming,Yang, Yajun,Hao, Xinyuan. 2014

[13]Osmotic responses of different strains of Steinernema carpocapsae. Yan, Xun,De Clercq, Patrick,Moens, Maurice,Yan, Xun,Han, Richou,Jones, John,Chen, Shulong,Moens, Maurice. 2011

[14]A gene family from Echinococcus granulosus differentially expressed in mature adult worms. Zhang, WB,Li, J,You, H,Zhang, ZZ,Turson, G,Loukas, A,McManus, DP. 2003

[15]Microarray analysis of gene expression in seeds of Brassica napus planted in Nanjing (altitude: 8.9 m), Xining (altitude: 2261.2 m) and Lhasa (altitude: 3658 m) with different oil content. Fu, San-Xiong,Qi, Cunkou,Cheng, Hao.

[16]Comparison of transcriptome under red and blue light culture of Saccharina japonica (Phaeophyceae). Wang, Wen-Jun,Wang, Fei-Jiu,Sun, Xiu-Tao,Liu, Fu-Li,Liang, Zhou-Rui.

[17]Differential gene expression in ulva prolifera under low light and low temperature conditions. Li, Youxun,Zhang, Xiaowen,Xu, Dong,Zhuang, Zhimeng,Ye, Naihao,Li, Youxun.

[18]Sex-biased miRNAs of yellow catfish (Pelteobagrus fulvidraco) and their potential role in reproductive development. Wang, Pingping,Wang, Le,Yang, Jian,Luan, Peixian,Zhang, Xiaofeng,Kuang, Youyi,Sun, Xiaowen. 2018

[19]Mutation in the gene encoding 1-aminocyclopropane-1-carboxylate synthase 4 (CitACS4) led to andromonoecy in watermelon. Ji, Gaojie,Zhang, Jie,Zhang, Haiying,Sun, Honghe,Gong, Guoyi,Shi, Jianting,Tian, Shouwei,Guo, Shaogui,Ren, Yi,Xu, Yong,Ji, Gaojie,Shen, Huolin,Gao, Junping. 2016

[20]Construction of a high-density linkage map and mapping of sex determination and growth-related loci in the mandarin fish (Siniperca chuatsi). Sun, Chengfei,Ye, Xing,Dong, Junjian,Zeng, Qingkai,Chen, Zhihang,Tian, Yuanyuan,Lu, Maixin,Niu, Yongchaox,Hu, Wushu,Zeng, Qingkai,Chen, Zhihang,Tian, Yuanyuan,Zhang, Jin. 2017

作者其他论文 更多>>

微信小程序