Development of 12 polymorphic EST-SSR for endangered fish, Hucho taimen (Pallas)

文献类型： 外文期刊

第一作者： Jun, Wang

作者： Jun, Wang;You-Yi, Kuang;Guang-Xiang, Tong;Jia-Sheng, Yin

作者机构：

关键词： cDNA library;expressed sequence tag derived simple sequence repeat markers: EST-SSR markers;CA-16 probe;heterozygosity;genetic diversity;fish breeding;polymorphism information content;breeding selection program

期刊名称：INDIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：0.316； 五年影响因子：0.366 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Hucho taimen (Pallas) is an indigenous and endangered cold-water fish in China. To access the genetic diversity of wild breeding parents of this species and promote its breeding selection program, 12 polymorphic EST-SSR markers were developed from its cDNA library by (CA)(16) probe and were analyzed on 66 wild breeding parents. The number of alleles per locus (Na), expected heterozygosity (He) and polymorphism information content (PIC) ranged from 2 to 9, 0.2019 to 0.8542 and 0.1802 to 0.8312 respectively. In total, a new and economical method for EST-SSR was established. The EST-SSR markers characterized in this study will strengthen our interpretation of Hucho taimen and facilitate its breeding selection program to improve the endangered statue of this species.

分类号： S8

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