Functional annotation of an expressed sequence tag library from Haliotis diversicolor and analysis of its plant-like sequences

文献类型： 外文期刊

第一作者： Jiang, Jing-Zhe

作者： Jiang, Jing-Zhe;Guo, Zhi-Xun;Su, You-Lu;Wang, Rui-Xuan;Wang, Jiang-Yong;Zhang, Wei;Cai, Chen-Chen

作者机构：

关键词： Small abalone;Haliotis diversicolor;Expressed sequence tag;Complementary DNA;Library;Plant-like gene

期刊名称：MARINE GENOMICS （ 影响因子：1.71； 五年影响因子：1.969 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The small abalone, Haliotis diversicolor, is a widely distributed and cultured species in the subtropical coastal area of China. To identify and classify functional genes of this important species, a normalized expressed sequence tag (EST) library, including 7069 high quality ESTs from the total body of H. diversicolor, was analyzed. A total of 4781 unigenes were assembled and 2991 novel abalone genes were identified. The GC content, codon and amino acid usage of the transcriptome were analyzed. For the accurate annotation of the abalone library, different influencing factors were evaluated. The gene ontology (GO) database provided a higher annotation rate (69.6%), and sequences longer than 800 bp were easily subjected to a BLAST search. The taxonomy of the BLAST results showed that lancelet and invertebrates are most closely related to abalone. Sixty-seven identified plant-like genes were further examined by reverse transcription–polymerase chain reaction (RT–PCR) and sequencing, only seven of these were real transcripts in abalone. Phylogenic trees were also constructed to illustrate the positions of two Cystatin sequences and one Calmodulin protein sequence identified in abalone. To perform functional classification, three different databases (GO, KEGG and COG) were used and 60 immune or disease-related unigenes were determined. This work has greatly enlarged the known gene pool of H. diversicolor and will have important implications for future molecular and genetic analyses in this organism.

分类号： Q3

  • 相关文献

[1]Construction of the BAC Library of Small Abalone (Haliotis diversicolor) for Gene Screening and Genome Characterization. Jiang, Likun,Xu, Jian,Jiang, Yanliang,Wang, Kai,Zhao, Zixia,Chen, Baohua,Zhao, Yunfeng,Xu, Peng,Jiang, Likun,Xu, Jian,Jiang, Yanliang,Wang, Kai,Zhao, Zixia,Chen, Baohua,Zhao, Yunfeng,Xu, Peng,You, Weiwei,Ke, Caihuan,Zhang, Xiaojun,Jiang, Likun,Chen, Baohua,Mahboob, Shahid,Al-Ghanim, Khalid A.,Mahboob, Shahid.

[2]Microdissection and Microcloning of Chromosome 5 in Gossypium arboreum. Peng Renhai,Liu Fang,Hu Xiao,Wang Chunying,Li Shaohui,Zhang Xiangdi,Wang Yuhong,Wang Kunbo.

[3]How A Library Provides Enterprises with Personalized Information Services under the Network Environment?. Zhang, Xiaohong,Zhou, Guomin,Song, Lirong,Li, Yanling. 2010

[4]Construction of a genomewide RNAi mutant library in rice. Wang, Lei,Zheng, Jie,Luo, Yanzhong,Xu, Tao,Zhang, Qiuxue,Zhang, Lan,Xu, Miaoyun,Zhang, Chunyi,Fan, Yunliu,Wan, Jianmin,Wang, Ming-Bo. 2013

[5]A deep-coverage BAC library for Ponkan mandarin and its further use in screening ethylene regulated genes. Wang, Miao,Tan, AnQun,Zhang, LingYun,Yang, JiaWei,Wei, ZhaoXin,Cheng, ChunZhen,Zhong, GuangYan,Zhou, ZhiQin,Zhong, GuangYan,Wang, Miao,Zhang, LingYun,Yang, JiaWei,Wei, ZhaoXin,Cheng, ChunZhen,Zhou, ZhiQin. 2010

[6]Development and characterization of twenty microsatellite markers for the endangered fish Luciobarbus capito. Geng Longwu,Lu Cuiyun,Tong Guangxiang,Li Chao,Xu Wei.

[7]Differential gene expression of cotton cultivar CCRI24 during somatic embryogenesis. Xiuming Wu,Fuguang Li#2;,Chaojun Zhang,Chuanliang Liu,Xueyan Zhang.

[8]Isolation, optimization, and functional analysis of the cDNA encoding transcription factor OsDREB1B in Oryza sativa L.. Qin, Qiu-lin,Liu, Jin-ge,Zhang, Zhen,Peng, Ri-he,Xiong, Ai-sheng,Yao, Quan-hong,Chen, Jian-min.

[9]Xuhuai Goat H-FABP Gene Clone, Subcellular Localization of Expression Products and the Preparation of Transgenic Mice. Yin, Yan-hui,Li, Bi-chun,Wei, Guang-hui,Zhu, Cai-ye,Li, Wei,Zhang, Ya-ni,Du, Li-xin,Cao, Wen-guang.

[10]Molecular Characterization and Expression Pattern of Two General Odorant Binding Proteins from the Diamondback Moth, Plutella xylostella. Zhang, Zhi-Chun,Wang, Man-Qun,Zhang, Guoan,Lu, Yao-Bin.

[11]Differential Gene Expression Analysis of Yunnan Red Pear, Pyrus Pyrifolia, During Fruit Skin Coloration. Zhang, Xiaodong,Yi, Qiong,Chen, Limei,Li, Kunzhi,Shu, Qun,Su, Jun,Allan, Andrew C..

[12]BIRC7 gene in channel catfish (Ictalurus punctatus): Identification and expression analysis in response to Edwardsiella tarda, Streptococcus iniae and channel catfish Hemorrhage Reovirus. Li, Min,Wang, Qi-Long,Chen, Song-Lin,Sha, Zhen-Xia,Li, Min,Liu, Yang.

[13]Construction, Characterization, and Expressed Sequence Tag (EST) Analysis of Normalized cDNA Library of Thermo-Photoperiod-Sensitive Genic Male Sterile (TPGMS) Wheat from Spike Developmental Stages. Yang, D.,Tang, Z. H.,Zheng, Y. L.,Tang, Z. H.,Zhang, L. P.,Zhao, C. P..

[14]Detection of abalone shrivelling syndrome-associated virus using loop-mediated isothermal amplification. Jiang, J-Z,Yang, R.,Zhang, H.,Liu, G-F,Su, Y-L,Wang, J-Y,Yang, R.,Zhang, H.,Zhu, Z-N,Wu, E-Y. 2014

[15]Nested PCR detection of abalone shriveling syndrome-associated virus in China. Jiang, Jing-Zhe,Luo, Li-Juan,Guo, Zhi-Xun,Liu, Guang-Feng,Su, You-Lu,Wang, Jiang-Yong,Liang, Ya-Yu,Zhuang, Jun. 2012

[16]Quantitative PCR detection for abalone shriveling syndrome-associated virus. Jiang Jing-Zhe,Zhang Han,Guo Zhi-Xun,Liu Guang-Feng,Su You-Lu,Wang Jiang-Yong,Zhu Zhen-Ni,Liang Ya-Yu,Zhang Han. 2012

[17]Genetic mapping and quantitative trait loci analysis of growth-related traits in the small abalone Haliotis diversicolor using restriction-site-associated DNA sequencing. Ren, Peng,You, Weiwei,Huang, Zekun,Guo, Qing,Chen, Nan,He, Peiru,Ke, Caihuan,Ren, Peng,Huang, Zekun,Guo, Qing,Chen, Nan,He, Peiru,Ke, Jinwei,Ke, Caihuan,Peng, Wenzhu,Peng, Wenzhu,Gwo, Jin-Chywan.

[18]Identification and involvement of ferritin in the response to pathogen challenge in the abalone, Haliotis diversicolor. He, Jian,Jiang, Jingzhe,Gu, Lu,Zhao, Manman,Wang, Ruixuan,Ye, Lingtong,Yao, Tuo,Wang, Jiangyong,Gu, Lu,Zhao, Manman.

[19]Molecular characterization of the genomic region harboring the BYDV-resistance gene Bdv2 in wheat. Xin, Z.,Zhang, Z.,Gao, L.,Ma, Q.,Liu, Y.. 2009

[20]Development and validation of 89 novel expressed sequence tag-derived microsatellite markers in blood clam, Tegillarca granosa. Teng, Shuangshuang,Fang, Jun,Cai, Yilong,Chai, Xueliang,Xiao, Guoqiang,Teng, Shuangshuang,Fang, Jun,Cai, Yilong,Chai, Xueliang,Xiao, Guoqiang,Teng, Shuangshuang,Fang, Jun,Cai, Yilong,Chai, Xueliang,Xiao, Guoqiang. 2017

作者其他论文 更多>>

微信小程序