Molecular and expression characterization of growth hormone/prolactin family genes in the Prenant's schizothoracin

文献类型： 外文期刊

第一作者： Li, Chuangju

作者： Li, Chuangju;Chen, Xihua;Zhang, Yan;Ye, Huan;Liu, Tao;Li, Chuangju;Zhang, Yan

作者机构：

关键词： mRNA;total RNA;peptide: amino acid residue;SMART cDNA: synthesis;Genbank sequence data;tissue distribution pattern

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The growth hormone/prolactin family genes are major participants in control of several complex physiologic processes, including growth, reproduction, and metabolism. In this study, total RNA was isolated from the pituitary of the Prenant's schizothoracin (Schizothorax prenanti), and SMART cDNA was synthesized from 100 ng total RNA. The full-length cDNAs of three GH/PRL family genes have been cloned and sequenced from the SMART cDNA library. The growth hormone of S. prenanti (SpGH) cDNA contains 1238 base pairs (bp) and encodes 215 amino acid (aa) residues; the prolactin (SpPRL) cDNA contains 1124 bp and encodes 209 aa residues; the cDNA sequence of somatolactin (SpSL) is 1290 bp in length, and encodes for a peptide of 234 aa residues. Although there is only about 22% aa sequence identity between the three deduced proteins, SpGH, SpPRL and SpSL, overall, the C-terminal region shows a higher identity. Multiple aa sequence alignments indicated the deduced SpGH, SpPRL and SpSL show high identities to that of goldfish and zebrafish, respectively. RT-PCR analysis revealed that the three hormones displayed different tissue distribution patterns. SpPRL transcripts were detected only in the pituitary of the Prenant's schizothoracin. SpSL was more widely distributed than SpPRL, high mRNA levels of SpSL were detected in the pituitary and low mRNA levels of SpSL were found in liver, kidney and heart. SpGH was expressed in nearly all tissues detected with the highest expression level in the pituitary.

分类号： Q7

  • 相关文献

[1]Cloning and expression of Toll-like receptors 1 and 2 from a teleost fish, the orange-spotted grouper Epinephelus coioides. Wei, You Chuan,Luo, Ting Rong,Wei, You Chuan,Pan, Ting Shuang,Chang, Ming Xian,Huang, Bei,Xu, Zhen,Nie, P.,Pan, Ting Shuang.

[2]Cloning and analysis of the full-length Rubisco large subunit (rbcL) cDNA from Ulva linza (Chlorophyceae, Chlorophyta). Ying, Cheng-Qi,Yin, Shun-Ji,Shen, Yi,He, Pei-Min,Ying, Cheng-Qi,Lin, Sen-Jie.

[3]Dissection of Myogenic Differentiation Signatures in Chickens by RNA-Seq Analysis. Li, Tingting,Zhang, Genxi,Wu, Pengfei,Liu, Qiuhong,Wang, Jinyu,Duan, Lian,Li, Guohui. 2018

[4]A novel pig gene, CPNE1, differentially expressed in the muscle tissues from Wujin pigs and Large White pigs. Liu Yonggang,Pu Shifei,Kong Chuisi. 2012

[5]Integration of lncRNA-miRNA-mRNA reveals novel insights into oviposition regulation in honey bees. Chen, Xiao,Chen, Chao,Shi, Wei,Liu, Zhiguang,Wang, Huihua,Guo, Haikun,Ma, Ce,Lu, Qian. 2017

[6]Dissecting the biological relationship between TCGA miRNA and mRNA sequencing data using MMiRNA-Viewer. Bai, Yongsheng,Ding, Lizhong,Rath, Ethan,Stuart, Gary,Bai, Yongsheng,Stuart, Gary,Baker, Steve,Jiang, Feng,Bai, Jenny M.,Wu, Jianghong,Jiang, Hui. 2016

[7]Identification and biochemical characterization of Laodelphax striatellus neutral ceramidase. Zhou, Y.,Lin, X. -W.,Zhang, Y. -R.,Huang, Y. -J.,Zhang, C. -H.,Yang, Q.,Yuan, J. -Q.,Cheng, J. -A.,Zhu, Z. -R.,Zhou, Y.,Lin, X. -W.,Zhang, Y. -R.,Huang, Y. -J.,Zhang, C. -H.,Yang, Q.,Yuan, J. -Q.,Cheng, J. -A.,Zhu, Z. -R.,Zhou, Y.,Xu, R.,Mao, C.,Li, H. -Y.,Yang, Q.. 2013

[8]Characterization and mapping of the bovine FBP1 gene. Guo, H.,Liu, W-S.,Takasuga, A.,Eyer, K.,Landrito, E.,Xu, S-Z.,Gao, X.,Ren, H-Y.. 2007

[9]Proliferation and bone-related gene expression of osteoblasts grown on hydroxyapatite ceramics sintered at different temperature. Wang, CY,Duan, YR,Markovic, B,Barbara, J,Howlett, CR,Zhang, XD,Zreiqat, H. 2004

[10]Nitrous oxide production and mRNA expression analysis of nitrifying and denitrifying bacterial genes under floodwater disappearance and fertilizer application. Riya, Shohei,Takeuchi, Yuki,Terada, Akihiko,Hosomi, Masaaki,Zhou, Sheng.

[11]Expression of Prolactin Receptor mRNA after Melatonin Manipulated in Cashmere Goats Skin during Cashmere Growth. Zhang, Wei,Zhu, Xiaoping,Jia, Zhihai,Yue, Chunwang,Kong, Xianghao,Du, Lixin.

[12]Cloning and Expression Analysis of the Smooth-Edge Seed Gene GSE-1 from Cucurbita pepo L. Guo, Shang,Guo, Shang,Guo, Shang,Tian, Ruxia,Feng, Zhiwei,Zhao, Chengfeng.

[13]BnHO1, a haem oxygenase-1 gene from Brassica napus, is required for salinity and osmotic stress-induced lateral root formation. Cao, Zeyu,Geng, Beibei,Xu, Sheng,Xuan, Wei,Nie, Li,Shen, Wenbiao,Cao, Zeyu,Geng, Beibei,Xu, Sheng,Xuan, Wei,Nie, Li,Shen, Wenbiao,Cao, Zeyu,Shen, Wenbiao,Liang, Yongchao,Cao, Zeyu,Guan, Rongzhan.

[14]Genome-wide transcriptome analysis in the ovaries of two goats identifies differentially expressed genes related to fecundity. Miao, Xiangyang,Luo, Qingmiao,Qin, Xiaoyu.

[15]Effects of temperature and dietary protein on the growth performance and IGF-I mRNA expression of juvenile mirror carp (Cyprinus carpio). Huang, J. F.,Xu, Q. Y.,Chang, Y. M.,Huang, J. F..

[16]Identification and expression analysis of the g-type and c-type lysozymes in grass carp Ctenopharyngodon idellus. Ye, Xing,Zhang, Lili,Tian, Yuanyuan,Tan, Aiping,Bai, Junjie,Li, Shengjie,Zhang, Lili.

[17]Effects of chromium picolinate supplementation on the growth, carcass quality and gene expression of beef during the finishing period. Liu Guifen,Wan Fachun,Song Enliang,Liu Xiaomu,Tan Xiuwen,Liu Zhenshan,Liu Guifen,Wan Fachun,Song Enliang,Liu Xiaomu,Tan Xiuwen,Liu Zhenshan.

[18]Expression profiles of key transcription factors involved in lipid metabolism in Beijing-You chickens. Fu, R. Q.,Liu, R. R.,Zhao, G. P.,Zheng, M. Q.,Chen, J. L.,Wen, J..

[19]Multiple splicing types of OsRIX4, an RAD21 homolog in rice (Oryza sativa L.). Dong HaiTao,Guo XiaoQin,Pei YanXi,Dai ChengEn,Fang YongQi,Tu QiChao,Zhuang JieYun,Zhao Dong,Zheng KangLe,Li DeBao.

[20]Influence of an m-type thioredoxin in maize on potyviral infection. Shi, Yan,Qin, Yanhong,Cao, Yanyong,Zhou, Tao,Fan, Zaifeng,Shi, Yan,Qin, Yanhong,Cao, Yanyong,Zhou, Tao,Fan, Zaifeng,Shi, Yan,Qin, Yanhong,Sun, Hu,Hong, Yiguo.

作者其他论文 更多>>

微信小程序