Scientific note on PCR inhibitors in the compound eyes of honey bees, Apis mellifera

文献类型： 外文期刊

第一作者： Boncristiani, Humberto

作者： Boncristiani, Humberto;Evans, Jay D.;Pettis, Jeff;Chen, Yanping;Li, Jilian

作者机构：

关键词： RNA;PCR inhibitor

期刊名称：APIDOLOGIE （ 影响因子：2.318； 五年影响因子：3.24 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The advent of new molecular technologies has revolutionized the diagnosis of pathogen-caused diseases in honey bees (Apis mellifera L.). Specifically, polymerase chain reaction (PCR)-based methods are widely applicable for the detection of difficult-to-grow pathogens such as viruses. In 1995, Stoltz et al. described specific reverse transcriptase-PCR (RT-PCR) primer pairs for the first detection of Kashmir bee virus infection in honey bees. Since then this methodology has offered a standard method for the detection and quantification of honey bee viruses (Stoltz et al. 1995).

分类号： Q969.9

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