Wide sugar substrate specificity of galactokinase from Streptococcus pneumoniae TIGR4

文献类型： 外文期刊

第一作者： Chen, Min

作者： Chen, Min;Chen, Lei-lei;Zou, Yang;Xue, Mengyang;Liang, Min;Jin, Lan;Guan, Wan-yi;Wang, Lei;Liu, Jun;Wang, Peng George;Chen, Min;Chen, Lei-lei;Zou, Yang;Xue, Mengyang;Liang, Min;Jin, Lan;Guan, Wan-yi;Wang, Lei;Liu, Jun;Wang, Peng George;Chen, Lei-lei;Shen, Jie;Wang, Wenjun;Wang, Peng George;Wang, Peng George

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关键词： Galactokinase;Streptococcus pneumoniae;Substrate specificity

期刊名称：CARBOHYDRATE RESEARCH （ 影响因子：2.104； 五年影响因子：2.192 ）

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收录情况： SCI

摘要： Galactokinases (GALK) have attracted significant research attention for their potential application in the enzymatic synthesis of unique sugar phosphates. The galactokinase (GalKSpe4) cloned from Streptococcus pneumoniae TIGR4 had a temperature optimum of 45 °C, and a pH optimum of 8.0. The substrate specificity and kinetics studies revealed that GalKSpe4 had moderate activity toward glucose, in contrast with very low or no activity observed in other previously reported GALKs. Most interestingly, GalKSpe4 exhibited activity for GalNAc, which had never been recorded in other GALKs found by now. This is the first time to report that bacterial GALK can recognize GalNAc.

分类号： O62

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