Single-step chromatography for simultaneous purification of C-phycocyanin and allophycocyanin with high purity and recovery from Spirulina (Arthrospira) platensis

文献类型： 外文期刊

第一作者： Yan, Shi-Gan

作者： Yan, Shi-Gan;Su, Hai-Nan;Zhang, Xi-Ying;Chen, Xiu-Lan;Zhou, Bai-Cheng;Zhang, Yu-Zhong;Zhu, Li-Ping;Yan, Shi-Gan

作者机构：

关键词： C-phycocyanin;Allophycocyanin;Anion exchange chromatography;pH gradient;Purification

期刊名称：JOURNAL OF APPLIED PHYCOLOGY （ 影响因子：3.215； 五年影响因子：3.612 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The cyanobacterium Spirulina (Arthrospira) platensis is a good source of phycobiliprotein purification. C-phycocyanin (C-PC) is the major phycobiliprotein, while allophycocyanin (APC) is less abundant in S. platensis. Previously reported methods for C-PC purification are only able to offer either high purity or high efficiency. This paper describes one-step anion exchange chromatography method with continuous pH gradient elution for simultaneous purification of C-PC and APC with high purity and highrecovery. Crude C-PC and APC were extracted and concentrated by ammonium sulfate fractionation at saturation of 25% and 60%, then purified on a DEAE-Sepharose Fast Flow chromatography column with continuous pH gradient elution from pH 5.0 to 3.6. After this single-step chromatography, C-PC and APC with high purity and recovery were simultaneously obtained. The purity ratios of C-PC and APC reached 5.59 (A620/A280) and 5.19 (A650/A280), respectively. Their purity was further demonstrated by electrophoresis and fluorescence emission spectroscopy. Moreover, the total recovery yield of pure C-PC and APC were 67.04% and 80.0%, representing 111.83 and 29.28 mg g~(-1) lyophilized weight, respectively. The obtained C-PC and APC remained stable over a pH rangeof 4–9. This purification method for high purity and recovery of C-PC and APC proved to be fairly efficient compared with previously reported methods.

分类号： Q94

  • 相关文献

[1]First biochemical characterization of a novel ribonuclease from wild mushroom Amanita hemibapha. Ma, Duanzheng,Wang, Hexiang,Sekete, Malota,Ma, Duanzheng,Wang, Hexiang,Wang, Bo,Ng, Tzibun. 2012

[2]Purification and characterization of a cis-epoxysuccinic acid hydrolase from Bordetella sp strain 1-3. Li, Xia,Lu, Hongbo,Ma, Xiaohang,Kai, Lei,Guo, Kangping,Zhao, Yuhua,Li, Xia,Xu, Tongcheng. 2010

[3]An acid-tolerant lectin coupled with high Hg2+ potentiated hemagglutination enhancing property purified from Amanita hemibapha var. ochracea. Ma, Duanzheng,Wang, He Xiang,Sekete, Malota,Ma, Duanzheng,Wang, He Xiang,Sekete, Malota,Wang, Bo,Gong, Zhiyuan,Ng, Tzi Bun. 2014

[4]Purification, characterization, and complement fixation activity of acidic polysaccharides from Tuber sinoaestivum. Zhang, Bo,Zhou, Jie,He, Xiaolan,Ye, Lei,Zou, Jie,Wu, Chenguang,Peng, Weihong,Zhang, Bo,Zou, Jie,Wu, Chenguang,Zhang, Xiaoping,Li, Juan.

[5]Molecular cloning of dihydroflavonol 4-reductase gene from grape berry and preparation of an anti-DFR polyclonal antibody. Zhang, Ping,Wen, Peng-Fei,Wan, Si-Bao,Wang, Wei,Pan, Qiu-Hong,Zhan, Ji-Chen,Huang, Wei-Dong,Zhang, Ping,Wen, Peng-Fei. 2008

[6]Isolation, Purification, and Immunological Activities of a Low-Molecular-Weight Polysaccharide from the Lingzhi or Reishi Medicinal Mushroom Ganoderma lucidum (Higher Basidiomycetes). Zhu, Lina,Luo, Xi,Tang, Qingjiu,Liu, Yanfang,Zhou, Shuai,Yang, Yan,Zhang, Jingsong,Zhu, Lina,Luo, Xi,Tang, Qingjiu,Liu, Yanfang,Zhou, Shuai,Yang, Yan,Zhang, Jingsong,Zhu, Lina,Luo, Xi. 2013

[7]Three phase partitioning for simultaneous purification of aloe polysaccharide and protein using a single-step extraction. Tan, Zhi-Jian,Wang, Chao-Yun,Yi, Yong-Jian,Wang, Hong-Ying,Zhou, Wan-Lai,Tan, Shi-Yong,Tan, Zhi-Jian,Li, Fen-Fang. 2015

[8]Preparation, purification and regeneration optimizing research of protoplasts from Rhizoctonia solani. Sun, Zhiguang,Qin, Peigang,Tang, Changqing,Fu, Rongtao,Liu, Yao,Li, Ping,Zheng, Aiping,Li, HaoJie,Feng, Haitao,Sun, Zhiguang,Qin, Peigang,Tang, Changqing,Fu, Rongtao,Liu, Yao,Li, Ping,Zheng, Aiping. 2012

[9]Ultrasonic-assisted extraction of sinomenine from Sinomenium acutum using magnetic ionic liquids coupled with further purification by reversed micellar extraction. Wang, Chaoyun,Yi, Yongjian,Zhou, Wanlai,Wang, Hongying,Tan, Zhijian,Wang, Chaoyun,Yi, Yongjian,Zhou, Wanlai,Wang, Hongying,Tan, Zhijian,Li, Qiao,Wu, Shuanggen,Li, Fenfang. 2017

[10]Purification and characterisation of a phosphatidylcholine-binding protein from duck Biceps femoris muscle. Wang, D. Y.,Zhang, M. H.,Liu, F.,Zhu, Y. Z.,Xu, W. M.. 2014

[11]A Simple Method for the Isolation and Purification of 2,4-Dihydroxy-7-Methoxy-2H-1,4-Benzoxazin-3(4H)-One (DIMBOA) from Maize (Zea mays L.) Seedlings. Liu Xin-gang,Dong Feng-shou,Xu Jun,Guo Li-qun,Kong Zhi-qiang,Tian Ying-ying,Zheng Yong-quan,Wu Yan-bin. 2013

[12]Purification and properties of lysozyme from a marine strain. Zhang, Xiu,Ren, Xian,Zhang, Xiu,Wang, Yue-Jun,Sun, Mi,Zou, Yan-Li,Wang, Qing-Yin,Wang, Wei. 2008

[13]Bacisubin, an antifungal protein with ribonuclease and hemagglutinating activities from Bacillus subtilis strain B-916. Chen, Zhiyi,Ng, T. B.,Zhang, Jie,Zhou, Mingguo,Song, Fuping,Lu, Fan,Liu, Youzhou. 2007

[14]Extraction, Purification and partial characterizations of Polysaccharides from Gracilaria lemaneiformis. Yang, Xianqing,Liu, Mingqiu,Qi, Bo,Li, Laihao,Deng, Jianchao,Hu, Xiao,Wu, Yanyan,Hao, Shuxian. 2014

[15]Protein Cyclization Enhanced Thermostability and Exopeptidase-Resistance of Green Fluorescent Protein. Zhao Zhonglin,Ma, Xin,Li, Liang,Zhang, Wei,Ping, Shuzhen,Lin, Min,Xu, Ming-Qun. 2010

[16]Comparison of cerato-platanin family protein BcSpl1 produced in Pichia pastoris and Escherichia coli. Liang, Yingbo,Qiu, Dewen,Yuan, Jingjing,Yang, Xiufen. 2017

[17]Biochemical characteristics of a novel protease from the basidiomycete Amanita virgineoides. Yuan, Xianghe,Wang, Hexiang,Yuan, Xianghe,Wang, Hexiang,Zhu, Mengjuan,Tian, Guoting,Zhao, Yongchang,Zhao, Liyan,Ng, Tzi Bun. 2017

[18]Purification and characterization of a psychrophilic catalase from Antarctic Bacillus. Wang, Wei,Sun, Mi,Zhang, Bin,Liu, Wanshun. 2008

[19]Isolation, Purification, Antioxidant and Immunological Activities in Vitro of Polysaccharide from Acanthopanacis Senticosi. Chen Ruizhan,Dong Hang,Meng Fanlei,Liu Zhiqiang. 2011

[20]Purification, characterization and immunomodulatory activity of water extractable polysaccharides from the swollen culms of Zizania latifolia. Wang, Mingchun,Zhao, Shiwei,Zhu, Peilei,Nie, Chenzhipeng,Ma, Shuping,Wang, Naifu,Du, Xianfeng,Zhou, Yibin,Wang, Mingchun,Zhao, Shiwei,Zhu, Peilei,Nie, Chenzhipeng,Ma, Shuping,Wang, Naifu,Du, Xianfeng,Zhou, Yibin,Zhu, Peilei. 2018

作者其他论文 更多>>

微信小程序