The Influence of RNAi Targeting of OsGA20ox2 Gene on Plant Height in Rice
文献类型: 外文期刊
第一作者: Qiao, Feng
作者: Qiao, Feng;Zhao, Kai-Jun;Qiao, Feng;Qiao, Feng;Zhao, Kai-Jun
作者机构:
关键词: gibberellin;GA3: gibberrelic acid;pCH12CK vector;pCH1CK vector;plant height;crop yield
期刊名称:PLANT MOLECULAR BIOLOGY REPORTER ( 影响因子:1.595; 五年影响因子:2.042 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: The rice height has been proposed to involve gibberellin biosynthesis. In order to regulate rice height, we suppressed expression of OsGA20ox2 by the double-stranded RNA interference (RNAi) method. For this purpose, we constructed two different RNAi vectors, pCH1CK and pCH12CK, then performed Agrobacterium-mediated transformation of rice varieties QX1 and Zhongzuo0201. The RNAi transgenic lines derived from pCH1CK and pCH12CK demonstrated a clear reduction in height compared with the control plants (P < 0.01). The height of dwarf lines from pCH1CK and pCH12CK were about 75-84% and 54-74% of that of the control plants, respectively. Transcripts for semi-dwarf lines from pCH1CK and pCH12CK were also reduced in comparison with the control plants. Analyses of seeds per panicle, 1,000-grain weight, and seed yield per plant suggested that the RNAi semi-dwarf lines showed stable grain yield compared with the wild-type plants. We further showed that the RNAi semi-dwarf lines could be restored to normal plant height by applying exogenous GA(3). The results also indicate that different fragment of the same RNAi target can be practically applied to produce different extent semi-dwarf of rice plants.
分类号: Q94
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