Development and characterization of 12 microsatellite markers for Johnius belengerii

文献类型： 外文期刊

第一作者： Ma, Chunyan

作者： Ma, Chunyan;Ma, Hongyu;Ma, Lingbo;Shen, Anglv;Gao, Lujiao

作者机构：

关键词： DNA;microsatellite marker: 12 isolates;alleles: 64 isolates;polymorphic microsatellite: 12 isolatesSequence DataJF766269: GenBank;EMBL;DDJB;nucleotide sequen;JF766270: GenBank;EMBL;DDJB;nucleotide sequence;JF766271: GenBank;EMBL;DDJB;nucleotide sequence;JF766272: GenBank;EMBL;DDJB;nucleotide sequence;JF766273: GenBank;EMBL;DDJB;nucleotide sequence;JF766274: GenBank;EMBL;DDJB;nucleotide sequence;JF766275: GenBank;EMBL;DDJB;nucleotide sequence;JF766276: GenBank;EMBL;DDJB;nucleotide sequence;JF766277: GenBank;EMBL;DDJB;nucleotide sequence;JF766278: GenBank;EMBL;DDJB;nucleotide sequence;JF766279: GenBank;EMBL;DDJB;nucleotide sequence;JF766280: GenBank;EMBL;DDJB;nucleotide sequence;heterozygosity;genetic diversity;Hardy-Weinberg equilibrium;fishery management;genetic background

期刊名称：CONSERVATION GENETICS RESOURCES （ 影响因子：0.973； 五年影响因子：0.946 ）

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收录情况： SCI

摘要： Twelve microsatellite DNA markers were developed for Johnius belengerii by 5' anchored PCR technique. A total of 64 alleles were identified in 30 individuals and the number of alleles per locus ranged from three to eight, with an average of 5.3. The observed and expected heterozygosity per locus ranged from 0.5000 to 0.9643 and from 0.5158 to 0.8413, respectively. Significant deviation from Hardy-Weinberg equilibrium was detected at three microsatellite loci after Bonferroni correction (P < 0.0042). No significant linkage disequilibrium was found between all pairs of 12 loci. These novel loci will be helpful for better understanding of genetic background of J. belengerii.

分类号： Q1

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