Single nucleotide mutation leading to an amino acid substitution in the variant Tik soybean Kunitz trypsin inhibitor (SKTI) identified in Chinese wild soybean (Glycine soja Sieb. & Zucc.)

文献类型: 外文期刊

第一作者: Wang, Ke-Jing

作者: Wang, Ke-Jing;Li, Xiang-Hua;Yamashita, Tetsuro;Takahata, Yoshihito

作者机构:

关键词: Kunitz-trypsin inhibitor;Protein polymorphism;Gene sequence;Wild soybean;Glycine soja

期刊名称:PLANT SYSTEMATICS AND EVOLUTION ( 影响因子:1.631; 五年影响因子:1.704 )

ISSN:

年卷期:

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收录情况: SCI

摘要: The wild soybean (Glycine soja), which is the progenitor of cultivated soybean (Glycine max), is expected to offer more information about genetic variability and more useful mutants for evolutionary research and breeding applications. Here, a total of 1,600 wild soybean samples from China were investigated for genetic variation with regard to the soybean Kunitz trypsin inhibitor (SKTI). A new mutant SKTI, Tik, was identified. It was found to be a Tia-derived codominant allele caused by a transversion point mutation from C to G at nucleotide +171, leading to an alteration of one codon (AAC -> AAG) and a corresponding amino acid substitution (Asn -> Lys) at the ninth residue. Upon examination of this variant and others previously found in wild soybeans, it became clear that SKTI has undergone high-level evolutionary differentiation. There were more abundant polymorphisms in the wild than in the cultivated soybean.

分类号: Q94

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