Isolation and characterization of a heme oxygenase-1 gene from Chinese cabbage

文献类型： 外文期刊

第一作者： Jin, Qi-Jiang

作者： Jin, Qi-Jiang;Cui, Wei-Ti;Han, Bin;Feng, Jian-Fei;Xu, Sheng;Shen, Wen-Biao;Jin, Qi-Jiang;Cui, Wei-Ti;Han, Bin;Feng, Jian-Fei;Xu, Sheng;Shen, Wen-Biao;Yuan, Xing-Xing

作者机构：

关键词： Abiotic stress responses;Chinese cabbage;Heme oxygenase-1;Prokaryotic expression in E. coli

期刊名称：MOLECULAR BIOTECHNOLOGY （ 影响因子：2.695； 五年影响因子：2.303 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： Heme oxygenase-1 (HO1) is a heme-catabolizing enzyme induced by a variety of stress conditions. This article described the cloning and characterization of BrHO1 gene which codes for a putative HO1 from Chinese cabbage (Brassica rapa subsp. pekinensis). BrHO1 consists of three exons and encodes a protein precursor of 32.3 kD with a putative N-terminal plastid transit peptide. The amino acid sequence of BrHO1 was 84% similar to Arabidopsis counterpart HY1. The three-dimensional structure of BrHO1 showed a high degree of structural conservation compared with the known HO1 crystal structures. Phylogenetic analysis revealed that BrHO1 clearly grouped with the HO1-like sequences. The recombinant BrHO1 protein expressed in Escherichia coli was active in the conversion of heme to biliverdin IXα (BV). Furthermore, the results of subcellular localization of BrHO1 demonstrated that BrHO1 gene product was most likely localized in the chloroplasts. BrHO1 was differently expressed in all tested tissues and could be induced upon osmotic and salinity stresses, cadmium (Cd) exposure, hydrogen peroxide (H_2O_2), and hemin treatments. Together, the results suggested that BrHO1 plays an important role in abiotic stress responses

分类号： Q7

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