Detection of ractopamine residues in pork by surface plasmon resonance-based biosensor inhibition immunoassay

文献类型： 外文期刊

第一作者： Zheng, Hong

作者： Zheng, Hong;Yang, Guo-Sheng;Lu, Xiao;Li, Xiu-Qing;Yuan, Xue-Xia;Deng, Li-Gang;Zhang, Hong;Wang, Wen-Zheng;Lu, Xiao;Li, Xiu-Qing;Yuan, Xue-Xia;Deng, Li-Gang;Zhang, Hong;Wang, Wen-Zheng;Yang, Guo-Sheng;Li, Hui;Meng, Meng;Xi, Ri-Mo;Aboul-Enein, Hassan Y.

作者机构：

关键词： ractopamine;pork;matrix effect;surface plasmon resonance;biosensor;UPLC-MS/MS

期刊名称：FOOD CHEMISTRY （ 影响因子：7.514； 五年影响因子：7.516 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： A surface plasmon resonance (SPR) biosensor inhibition immunoassay for determination of ractopamine (Rac) residue in pork was constructed by immobilising Rac derivative on the SPR-2004 biosensor chip. After extraction with perchloric acid, pork sample was cleaned by ethyl acetate and analyzed by SPR-2004 biosensor. The limit of detection (LOD) was 0.6 ug/kg for pork sample. Recoveries of Rac were higher than 80% with relative standard deviations below 10%. Although the same pretreatment was applied for both the UPLC-MS/MS and the biosensor, the biosensor showed little matrix interference by constructing pure solution and matrix-match calibration curves. Compared with Biacore Q made by Biacore AB, SPR-2004 biosensor made by Chinese Academy of Sciences which is of low cost and showed minimal matrix interferences. Accordingly, this biosensor was a promising screening instrument to be used for detection of ractopamine in supermarkets, food factories and food regulatory organisations.

分类号： TS2

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