Production of triploid plants of papaya by endosperm culture

文献类型： 外文期刊

第一作者： Sun, De-Quan

作者： Sun, De-Quan;Lu, Xin-Hua;Mo, Yi-Wei;Xie, Jiang-Hui;Liang, Guo-Lu;Guo, Qi-Gao

作者机构：

关键词： Carica papaya;Endosperm culture;Callus induction;Regeneration;Triploids

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Triploid papaya (Carica papaya) plants were obtained by immature endosperm culture. Visible callusing of the endosperm occurred 21 days after initiation of cultures. A continuously growing callus was observed and a maximum of 68.7% of callus induction frequency was obtained when immature endosperm with embryo was cultured on Murashige and Skoog (MS) medium containing 6.0 mu M 2,4-dichlorophenoxyacetic acid (2,4-D), 2.5 mu M alpha-naphthaleneacetic acid (NAA) and 4.0 mu M 6-furfurylamino purine (Kn). Shoot buds were produced when the callus was subcultured on a medium supplemented with 6-benzyladenine (BA) or thidiazuron (TDZ) along with NAA. Shoots were detached from the callus and transferred to the elongation medium supplemented with indole-3-acetic acid (IAA) and BA. The combination of 3.0 mu M IAA and 1.5 mu M BA was the best in terms of the number of cultures (93.8%) showing axillary shoot proliferation. The addition of 2.0 mu M indole-3-butyric acid (IBA) to the 1/2 MS medium was most effective at inducing root formation with 90% of the shoots developing four to five roots. Healthy rooted plantlets were transferred to pots containing sterilized bed soil and perlite (3:1) mixture in the greenhouse and 78% of the micropropagated plants survived transplantation. The leaves from endosperm-derived plants showed larger stomata and more chloroplasts in guard cells than that from the parent plants. Over 75% of the endosperm-derived plants were triploid with chromosome number 2n = 3x = 27.

分类号： Q942

  • 相关文献

[1]ELEVATED ENDOGENOUS ISOPENTENYL ADENINE CONTENT IS CORRELATED WITH AN EXTREMELY SHOOTY RICE PHENOTYPE. ZHU, YX,QIN, RZ,SHAN, XY,CHEN, ZL.

[2]Inheritance of anthocyanin content in the ripe berries of a tetraploid x diploid grape cross population. Liang, Zhenchang,Li, Shaohua,Liang, Zhenchang,Wu, Benhong,Liang, Zhenchang,Wu, Benhong,Sang, Min,Ma, Aihong,Zhao, Shengjian,Zhong, Gan-Yuan.

[3]Collaborative Ring Trial of the Papaya Endogenous Reference Gene and Its Polymerase Chain Reaction Assays for Genetically Modified Organism Analysis. Wei, Jiaojun,Guo, Jinchao,Zhang, Dabing,Yang, Litao,Li, Feiwu,Li, Xiang,Xu, Junfeng,Wu, Gang.

[4]Characterization of a root-specific beta-thioglucoside glucohydrolase gene in Carica papaya and its recombinant protein expressed in Pichia pastoris. Wang, Meng,Li, Dingqin,Sun, Xuepiao,Nong, Han,Zhang, Jiaming,Zhu, Yun Judy,Nong, Han. 2009

[5]Properties of soluble dietary fiber-polysaccharide from papaya peel obtained through alkaline or ultrasound-assisted alkaline extraction. Zhang, Weimin,Zeng, Guanglin,Pan, Yonggui,Chen, Wenxue,Chen, Haiming,Huang, Wuyang,Li, Yuansong,Zhang, Weimin,Chen, Haiming.

[6]Efficient somatic embryogenesis in sugar beet (Beta vulgaris L.) breeding lines. Zhang, Chun-Lai,Chen, Dong-Fang,Kubalakova, Marie,Zhang, Jian,Scott, Nigel W.,Elliott, Malcolm C.,Slater, Adrian,Zhang, Chun-Lai,Chen, Dong-Fang,Kubalakova, Marie,Zhang, Jian.

[7]High Efficiency Shoot Regeneration from Leaf Explants of Taishan Sour Jujube (Zizyphus spinosus Hu.). Sun, H.,Sun, Q.,Zhou, G.,Liu, Q.,Xin, L.. 2009

[8]High-Efficiency Plant Regeneration from Immature Inflorescence Derived Callus Cultures of Two Phenotypically Distinct Accessions of Centipedegrass (Eremochloa ophiuroides). Ma, Jingjing,Wang, Yi,Zong, Junqin,Zhang, Bing,Chen, Jingbo,Li, Dandan,Li, Ling,Guo, Hailin,Liu, Jianxiu,Li, Jianjian,She, Jianming. 2018

[9]Comparison of dynamic changes of endogenous hormones between calli derived from mature and immature embryos of maize. Wang, Henning,Zhang, Jinwen,Jia, Xiaoxia,Liang, Huiguang,Kong, Weiping,Zhang, Xinhui. 2011

[10]Establishment of a highly efficient regeneration method from the scraped broken embryo of mature barley seed. Hua, Wei,Zhu, Jinghuan,Shang, Yi,Wang, Junmei,Jia, Qiaojun,Lin, Feng,Yang, Jianming. 2013

[11]Shoot organogenesis and plant regeneration in Agave hybrid, No. 11648. Zhang, Yan-Mei,Li, Jun-Feng,Lu, Jun-Ying,Zhou, Wen-Zhao,Li, Xin,Chen, Zhi. 2013

[12]Callus induction and plant regeneration in Alternanthera philoxeroides. Gao, Jianming,Li, Jinzhi,Luo, Caibo,Yin, Liang,Li, Shengchun,Yang, Guangxiao,He, Guangyuan,Gao, Jianming,Li, Jinzhi.

[13]Rapid and highly efficient callus induction and plant regeneration in the starch-rich duckweed strains of Landoltia punctata. Huang, Meng,Fu, Lili,Sun, Xuepiao,Zhang, Jiaming,Di, Rong.

[14]Low temperature treatments of rice (Oryza sativa L.) anthers changes polysaccharide and protein composition of the anther walls and increases pollen fertility and callus induction. Tian, Q. Q.,Li, X.,Fang, X. W.,Tian, Q. Q.,Lu, C. M.,Li, X..

[15]High efficiency in vitro plant regeneration from epicotyl explants of Chinese peanut cultivars. Shan, Lei,Tang, Guiying,Xu, Pingli,Liu, Zhanji,Bi, Yuping,Shan, Lei,Tang, Guiying,Xu, Pingli,Liu, Zhanji,Bi, Yuping,Shan, Lei,Tang, Guiying,Xu, Pingli,Liu, Zhanji,Bi, Yuping.

[16]Relationships between endogenous hormonal content and direct somatic embryogenesis in Watermelon (Citrullus lanatus) cotyledons. Zhang, Hui Jun,Wang, Qing,Wang, Qing. 2015

[17]Preparation, purification and regeneration optimizing research of protoplasts from Rhizoctonia solani. Sun, Zhiguang,Qin, Peigang,Tang, Changqing,Fu, Rongtao,Liu, Yao,Li, Ping,Zheng, Aiping,Li, HaoJie,Feng, Haitao,Sun, Zhiguang,Qin, Peigang,Tang, Changqing,Fu, Rongtao,Liu, Yao,Li, Ping,Zheng, Aiping. 2012

[18]Disease-resistant transgenic adzuki bean plants obtained through an efficient transformation system. Chen, Huatao,Chen, Xin,Gu, Heping,Yuan, Xingxing,Zhang, Hongmei,Cui, Xiaoyan. 2012

[19]Regeneration and transformation of Crambe abyssinica. Qi, Weicong,Tinnenbroek-Capel, Iris E. M.,Schaart, Jan G.,Cheng, Jihua,Visser, Richard G. F.,Van Loo, Eibertus N.,Krens, Frans A.,Qi, Weicong,Huang, Bangquan,Cheng, Jihua. 2014

[20]Effects of iodine and light intensity on micropropagation of purple coneflower (Echinacea purpurea (L.) Moench). Chen Xiaolu,Li Dongliang,Chen Xiaolu,Yang Yuesheng. 2016

作者其他论文 更多>>

微信小程序