Biodegradation of methyl parathion and p-nitrophenol by a newly isolated Agrobacterium sp strain Yw12

文献类型： 外文期刊

第一作者： Wang, Shenghui

作者： Wang, Shenghui;Yan, Yanchun;Wang, Shenghui;Zhang, Chen

作者机构：

关键词： Methyl parathion;p-Nitrophenol;Degradation;Agrobacterium sp.;Response surface methodology

期刊名称：BIODEGRADATION （ 影响因子：3.909； 五年影响因子：3.578 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Strain Yw12, isolated from activated sludge, could completely degrade and utilize methyl parathion as the sole carbon, phosphorus and energy sources for growth in the basic salt media. It could also completely degrade and utilize p-nitrophenol as the sole carbon and energy sources for growth in the minimal salt media. Phenotypic features, physiological and biochemical characteristics, and phylogenetic analysis of 16S rRNA sequence showed that this strain belongs to the genus of Agrobacterium sp. Response surface methodology was used to optimize degradation conditions. Under its optimal degradation conditions, 50 mg l(-1) MP was completely degraded within 2 h by strain Yw12 and the degradation product PNP was also completely degraded within 6 h. Furthermore, strain Yw12 could also degrade phoxim, methamidophos, chlorpyrifos, carbofuran, deltamethrin and atrazine when provided as the sole carbon and energy sources. Enzymatic analysis revealed that the MP degrading enzyme of strain Yw12 is an intracellular enzyme and is expressed constitutively. These results indicated that strain Yw12 might be used as a potential and effective organophosphate pesticides degrader for bioremediation of contaminated sites.

分类号： R

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