Development and use of novel SSR markers for molecular genetic diversity in Italian millet (Setaria italica L.)
文献类型: 外文期刊
第一作者: Zhao, Weiguo
作者: Zhao, Weiguo;Kwon, Soon-Wook;Park, Yong-Jin;Zhao, Weiguo;Lee, Gi-An;Ma, Kyung-Ho;Lee, Myung-Chul;Kwon, Soon-Wook;Park, Yong-Jin
作者机构:
关键词: Genetic diversity;Heterozygosity;Italian millet;Simple sequence repeat (SSR) markers;Setaria italica
期刊名称:GENES & GENOMICS ( 影响因子:1.839; 五年影响因子:1.329 )
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收录情况: SCI
摘要: Italian millet is a commercially important grain crop. Nineteen polymorphic simple sequence repeat (SSR) markers, developed through construction of an SSR-enriched library from genomic DNA of Italian millet (Setaria italica L., P. Beauv.), were used for assessment of molecular genetic diversity against 40 accessions of S. italica. In total, 85 alleles were detected, with an average of 4.5 alleles per locus. The average gene diversity and polymorphism information content (PIC) values were 0.412 and 0.376, ranging from 0.02 to 0.88 and from 0.02 to 0.87, respectively. Values for observed (Ho) and expected (He) heterozygosities ranged from 0 to 0.73 and from 0.03 to 0.89, respectively. Nine loci deviated from Hardy-Weinberg equilibrium. The mean similarity coefficient among accessions was 0.6593. Based on the UPGMA algorithm, six different groups were successfully identified. In this clustering analysis, all Korean accessions grouped in one cluster, indicating that Korean accessions are genetically quite distinct from other introduced accessions. These newly developed microsatellite markers should be very useful tools for several genetic studies, including an assessment of diversity and population structure in Italian millet.
分类号: Q
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