Characterization of the Hormone and Stress-Induced Expression of FaRE1 Retrotransposon Promoter in Strawberry
文献类型: 外文期刊
第一作者: He, Ping
作者: He, Ping;Ma, Yue;Dai, Hongyan;Liu, Yuexue;Li, He;Zhang, Zhihong;He, Ping;Li, Linguang;Zhao, Guiling
作者机构:
关键词: FaRE1 retrotransposon;Strawberry;Promoter;Stress-induced expression
期刊名称:JOURNAL OF PLANT BIOLOGY ( 影响因子:2.434; 五年影响因子:2.455 )
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收录情况: SCI
摘要: Retrotransposons are the most abundant mobile elements in the plant genome and seem to play an important role in genome reorganization induced by environmental challenges. Their success in this function depends on the ability of their promoters to regulate plant adaptation to biotic and abiotic stresses. In this study, the promoter region of FaRE1 was amplified in the strawberry genome, and promoter::GUS fusion was constructed. We produced transgenic strawberry plants carrying FaRE1 promoter::GUS-fusion genes, and monitored GUS reporter activity. Histochemical and fluorimetric GUS analysis these plants showed the characteristics of the FaRE1 promoter were activated by either hormones treatments with ABA, NAA, and 2,4-D or cold stress. In addition, we found the GUS reporter was activated in the leaves of transgenic strawberry plants using 5-azaC. These results suggest that the promoter of FaRE1 may act as different signal transduction pathways, allowing FaRE1 retrotransposon to be activated in response to multiples challenges.
分类号: Q94
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