Genomic structure, polymorphism and expression analysis of the growth hormone (GH) gene in female and male Half-smooth tongue sole (Cynoglossus semilaevis)

文献类型： 外文期刊

第一作者： Ma, Qian

作者： Ma, Qian;Liu, ShuFang;Zhuang, ZhiMeng;Lin, Lin;Sun, ZhongZhi;Liu, ChangLin;Ma, Hui;Tang, QiSheng;Ma, Qian;Lin, Lin;Su, YongQuan;Ma, Hui

作者机构：

关键词： Growth hormone;Half-smooth tongue sole;Microsatellite marker;MRNA expression

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

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年卷期：

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收录情况： SCI

摘要： Growth hormone (GH) is a polypeptide which is an important regulator of development and somatic growth in teleosts, and may be associated with the mechanisms which drive sexual growth dimorphism in the Half-smooth tongue sole (Cynoglossus semilaevis). In this study, the full length gh cDNA was cloned from C. semilaevis by homology cloning and the rapid amplification of cDNA ends polymerase chain reaction (RACE-PCR). The full-length gh cDNA is 826. bp and contains an open reading frame (ORF) of 603. bp encoding a protein of 200 amino acids (AA). The precursor of gh consists of a 17 amino-acid signal peptide followed by a 183 amino-acid mature polypeptide. GH gene sequences obtained from female and male adults consist of 3428. bp and 3371. bp, respectively, each of which includes six exons and five introns, and the difference in the GH gene size was mainly caused by the microsatellites. When 14 tissues from females, normal males and extra-large male adults were analyzed for sex-specific tissue expression, the gh mRNA was found to be predominantly expressed in the pituitary, and the expression levels in females were 3.6 times as much as those in normal males, while the mRNA expression in extra-large males was 1.7 times as much as those in normal males. Sex differences in gh mRNA expression during development were also examined by using a full-sib family of C. semilaevis, and the gh mRNA was detected at all of the 12 time points sampled from 10 to 380. days-old. A significant increase in gh mRNA was detected starting in 80. day old fish and was then followed by a drop to very low levels starting at 230. day old fish. Differential expression indicated that the gh expression level in females was significantly higher than males (P< 0.01) at all of the stages except for 10. days-old. Two microsatellite loci were identified in the second intron of the GH gene. Using these two polymorphic markers to genotype 224 individuals, there was no significant difference between the females and males in the Bohai Sea, the Yellow Sea and the hatchery samples.

分类号： R394

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