Screening of a xylanase high-producing strain and its rapid separation and purification

文献类型: 外文期刊

第一作者: Liu, Zhengchu

作者: Liu, Zhengchu;Duan, Shengwen;Zheng, Ke;Feng, Xiangyuan;Cheng, Lifeng;Shi, Jun;Dai, Xiaoyang;Zhang, Juzuo;Xu, Junfei

作者机构:

关键词: Xylanase;High-producing strain;Rapid purification

期刊名称:ANNALS OF MICROBIOLOGY ( 影响因子:2.112; 五年影响因子:2.151 )

ISSN:

年卷期:

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收录情况: SCI

摘要: In this research, the xylanase high-producing strain of BE-91 (Bacillus subtilis) was selected. The enzyme activity in the fermentation liquor of BE-91 at 8 h reached 408 U/mL, which was 3.4 times higher than that of strain ACCC 10243. The xylanase was purified from BE-91 fermentation liquor with the ultrafiltration and gel chromatography, and its enzyme activity was up to 28,454 U/mg. Its recovery was above 69%, and the purification multiple of the enzyme activity was up to 18 times. The molecular weight of the purified xylanase was 22.54 kDa assayed with SDS-PAGE. The K_m and V_(max) were 0.5 mg/mL and 533 μmol/(min mL), respectively. The stabilizing pH and optimal pH of the xylanase were 4.6~6.4 and 5.8, respectively. And when the pH was 5.8, the stabilizing temperature and optimal temperature of the xylanase were 0°C~65°C and 60°C, respectively. Therefore it was considered that the strain BE-91 could be applied to the industrial production of xylanase.

分类号: Q93

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