A novel dendrimeric peptide induces high level neutralizing antibodies against classical swine fever virus in rabbits

文献类型： 外文期刊

第一作者： Li, Guo-Xin

作者： Li, Guo-Xin;Zhou, Yan-Jun;Yu, Hai;Li, Ling;Wang, Ya-Xin;Tong, Wu;Hou, Jun-Wei;Xu, Yan-Zhao;Zhu, Jian-Ping;Xu, Ao-Tian;Tong, Guang-Zhi

作者机构：

关键词： Classical swine fever virus;Dendrimeric peptide;Neutralizing epitope;T helper cell epitope;Marker vaccine

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

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收录情况： SCI

摘要： The amino acid sequence (TAVSPTTLR, 829-837 aa) on the glycoprotein E2 of classical swine fever virus (CSFV) is a conserved and linear neutralizing epitope. In the present study, two peptides were constructed based the core sequence of this neutralizing epitope, the dendrimeric peptide (Th-B-4) containing four copies of B cell epitope fused to one copy of promiscuous T helper (Th) cell epitope and the peptide Th-B containing a single copy of B cell epitope fused to one copy of Th cell epitope. The dendrimeric peptide Th-B-4 elicited high titers of neutralizing antibodies as detected in an indirect ELISA, blocking ELISA and neutralization test and induced a complete protection against CSFV C strain in rabbits. The Th-B elicited low titers of neutralizing antibodies and did not induce a protection in rabbits. These results suggest that the dendrimeric peptide Th-B-4 may be a promising marker vaccine candidate against CSFV and the multimerization is a requirement for development of a peptide vaccine

分类号： S8

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