An outbreak of sheep pox associated with goat poxvirus in Gansu province of China

文献类型： 外文期刊

第一作者： Yan, Xin-Min

作者： Yan, Xin-Min;Chu, Yue-Feng;Wu, Guo-Hua;Zhao, Zhi-Xun;Li, Jian;Zhu, Hai-Xia;Zhang, Qiang

作者机构：

关键词： Sheep pox;Goat poxvirus;Characterization;P32;RPO30

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

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收录情况： SCI

摘要： Three strains of Capripoxviruses (CaPVs) were isolated from an outbreak of sheep pox in Gansu province of China. They were analyzed by P32 gene-based molecular methods and a species-specific PCR based on the RPO30 gene. Two bands which are specific to goat poxvirus (GTPV) were observed after the PCR products of P32 gene were digested with the endonuclease of Hinf I. Moreover, an amplicon of 172 bp, which is specific to GTPV, was amplified from the viruses by using the RPO30 gene-based PCR. Sequence analysis of the P32 genes showed that three nucleotide bases for coding residue of aspartic acid which are located at 163-165 position of P32 gene of sheep poxvirus (SPPV) were absent, and six single nucleotide substitutions which are characteristic of GTPV were present. The viruses were genetically closer to GTPV strains and clustered into the GTPV branch of the phylogenetic tree constructed on the basis of the P32 gene. The results characterized the isolated viruses as GTPV. It is the first report of an outbreak of sheep pox associated with GTPV in China. (c) 2011 Elsevier B.V

分类号： S8

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