Optimisation of preparation conditions and properties of phytosterol liposome-encapsulating nattokinase

文献类型： 外文期刊

第一作者： Dong, Xu-Yan

作者： Dong, Xu-Yan;Wang, Zhan;Zhao, Yuan-Di;Dong, Xu-Yan;Kong, Fan-Pi;Yuan, Gang-You;Wei, Fang;Jiang, Mu-Lan;Li, Guang-Ming;Chen, Hong

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关键词： Encapsulating properties;Liposome;Nattokinase;Phytosterol preparation

期刊名称：NATURAL PRODUCT RESEARCH （ 影响因子：2.861； 五年影响因子：2.572 ）

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收录情况： SCI

摘要： Phytosterol liposomes were prepared using the thin film method and used to encapsulate nattokinase (NK). In order to obtain a high encapsulation efficiency within the liposome, an orthogonal experiment (L9 (3) 4) was applied to optimise the preparation conditions. The molar ratio of lecithin to phytosterols, NK activity and mass ratio of mannite to lecithin were the main factors that influenced the encapsulation efficiency of the liposomes. Based on the results of a single-factor test, these three factors were chosen for this study. We determined the optimum extraction conditions to be as follows: a molar ratio of lecithin to phytosterol of 2 : 1, NK activity of 2500U mL -1 and a mass ratio of mannite to lecithin of 3 : 1. Under these optimised conditions, an encapsulation efficiency of 65.25% was achieved, which agreed closely with the predicted result. Moreover, the zeta potential, size distribution and microstructure of the liposomes prepared were measured, and we found that the zeta potential was -51±3mV and the mean diameter was 194.1 nm. From the results of the scanning electron microscopy, we observed that the phytosterol liposomes were round and regular in shape and showed no aggregation.

分类号： R9

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