HPLC-MS/MS enantioseparation of triazole fungicides using polysaccharide-based stationary phases

文献类型: 外文期刊

第一作者: Zhang, Hu

作者: Zhang, Hu;Wang, Minghua;Zhang, Hu;Wang, Minghua;Zhang, Hu;Qian, Mingrong;Wang, Xinquan;Wang, Xiangyun;Xu, Hao;Wang, Qiang;Zhang, Hu;Qian, Mingrong;Wang, Xinquan;Wang, Xiangyun;Xu, Hao;Wang, Qiang;Zhang, Hu;Qian, Mingrong;Wang, Xinquan;Wang, Xiangyun;Xu, Hao;Wang, Qiang

作者机构:

关键词: Chiral separation;HPLC-MS/MS;Polysaccharide-based stationary phases;Reversed phase conditions;Triazole fungicides

期刊名称:JOURNAL OF SEPARATION SCIENCE ( 影响因子:3.645; 五年影响因子:2.943 )

ISSN:

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收录情况: SCI

摘要: The enantiomeric separation of 21 triazole fungicides was carried out on four polysaccharide-derived chiral stationary phases in the reversed phase separation mode using high performance liquid chromatography coupled with tandem mass spectrometry. All fungicides were detected in electrospray ionization (ESI) positive mode with selected reaction monitoring (SRM). Complete enantioseparation was achieved for 21 fungicides except for difenoconazole based on cellulose tris (3,5-dimethylphenylcarbamate) and cellulose tris (3-chloro-4-methylphenyl carbamate) columns by optimizing experimental conditions including mobile phase and column temperature. Mobile phase was 0.1% formic acid aqueous solution mixed with methanol or acetonitrile in different proportions. Among all the fungicides, 15 with two enantiomers and three with four stereoisomers (bitertanol, bromuconazole, and cyproconazole) were successfully separated at 25°C. Enantioseparation for the other three fungicides (propiconazole, triadimenol, and difenoconazole) with four stereoisomers could be achieved by changing the column temperature from 10 to 40°C. Propiconazole and triadimenol were enantioseparated on baseline at 40 and at 35°C, respectively, and difenoconazole was enantioseparated partially with the R _s > 1.1 at 25°C. Moreover, linearities and limits of detection (LODs) of 21 fungicides except for difenoconazole were studied, showing coefficients of determination (R ~2) higher than 0.99 and LODs lower than 2.5 μg/L.

分类号: O6

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