Next-generation small RNA sequencing for microRNAs profiling in Apis mellifera: comparison between nurses and foragers

文献类型： 外文期刊

第一作者： Liu, F.

作者： Liu, F.;Li, Z.;Li, W.;Li, L.;Pan, J.;Zhang, S.;Miao, Y.;Chen, S.;Su, S.;Peng, W.;Zhang, S.

作者机构：

关键词： Apis mellifera;microRNA;deep sequencing;honey bee

期刊名称：INSECT MOLECULAR BIOLOGY （ 影响因子：3.585； 五年影响因子：3.215 ）

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收录情况： SCI

摘要： MicroRNAs (miRNAs) are endogenous small non-coding RNAs regulating gene expression in animals and plants. To find some differentially expressed miRNAs that may be associated with age-dependent behavioural changes in honey bees (Apis mellifera), we applied next-generation high-throughput sequencing technology to detect small RNAs in nurses and foragers. Our results showed that both nurses and foragers had a complicated small RNA population, and the length of small RNAs varied, 22 nucleotides being the predominant length. Combining deep sequencing and bioinformatic analysis, we discovered that nine known miRNAs were significantly different between nurses and foragers (P < 0.01; absolute value of fold-change =1). Some of their target genes were related to neural function. Moreover, 67 novel miRNAs were identified in nurses and foragers. Ame-miR-31a and ame-miR-13b were further validated using quantitative reverse-transcription PCR assays. The present study provides new information on the miRNA abundance of honey bees, and enhances our understanding of miRNA function in the regulation of honey bee development.

分类号： R47

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