Rapid detection of duck hepatitis virus type-1 by reverse transcription loop-mediated isothermal amplification

文献类型： 外文期刊

第一作者： Song, Cuiping

作者： Song, Cuiping;Wan, Hongquan;Yu, Shengqing;Han, Xiangan;Qiu, Xusheng;Hu, Qinghai;Tan, Lei;Ding, Chan

作者机构：

关键词： Duck hepatitis;Reverse transcription loop-mediated isothermal amplification (RT-LAMP);Sensitivity;Specificity

期刊名称：JOURNAL OF VIROLOGICAL METHODS （ 影响因子：2.014； 五年影响因子：2.001 ）

ISSN：

年卷期：

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收录情况： SCI

摘要： A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for the detection of duck hepatitis virus type-1 (DHV-1) was established. Using primers specific to the highly conserved 3D gene of DHV-1, the developed RT-LAMP assay detected the viral RNA of DHV-1 extracted from both allantoic fluid and liver samples of infected ducks. The assay is as sensitive as RT-PCR, and shows no cross-reaction with other common avian viral and bacterial pathogens. In addition to detection via ethidium bromide staining following gel electrophoresis, naked-eye observation after staining with SYBR Green I dye can be used to detect RT-LAMP products; this enables field application of this assay. The findings demonstrate that RT-LAMP can serve as a helpful tool for the detection and surveillance of DHV-1 in the poultry industry. (C) 2012 Elsevier B.V

分类号： R37

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