Mutations in the genome of the highly pathogenic porcine reproductive and respiratory syndrome virus potentially related to attenuation
文献类型: 外文期刊
第一作者: Leng, Xue
作者: Leng, Xue;Li, Zhenguang;Wang, Fengxue;Wang, Wei;Zhang, Xiuhua;Wu, Hua;Xia, Mingqi;Li, Xiyu;Wu, Hua
作者机构:
关键词: HP-PRRSV;Genetic variation;Attenuation;Deletion
期刊名称:VETERINARY MICROBIOLOGY ( 影响因子:3.293; 五年影响因子:3.599 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: A live-attenuated highly pathogenic porcine reproductive and respiratory syndrome (HP-PRRS) virus (HP-PRRSV) TJM vaccine strain was derived from the HP-PRRSV TJ strain by passage 92 times in the African green monkey kidney epithelial cell line Marc-145. We found that the virulence of the TJ strain to piglets was decreased greatly from passage 19. To identify mutations associated with attenuation of the TJM vaccine strain, we determined the nucleotide changes that arose during Marc-145 passage of the HP-PRRSV TJ virus. The TJM strain contains a 360 nucleotide (120 amino acids) deletion and a 118 nucleotide mutation that resulted in 48 amino acid changes. Analysis of the complete nucleotide sequences of intermediate passage-level viruses F19, F46 and F78 showed that 31 (64.6%) of the 48 amino acid mutations occurred in F19, 7 (14.6%) occurred in F46, 7 (14.6%) occurred in F78 and 3 (6.3%) occurred in F92. The 120 amino acid deletion occurred from F19 to TJM. Therefore, we hypothesized that the 31 amino acid mutations distributed in nsp1 beta, nsp2-nsp5, nsp7, nsp9, nsp10, GP4 and GP5 and the continuous 120 amino acid deletion in the nsp2 region from F19 provide a strong potential molecular basis for the observed attenuated phenotype. (C) 2011 Elsevier B.V
分类号: S8
- 相关文献
作者其他论文 更多>>
-
Whole genome and transcriptome analyses in dairy goats identify genetic markers associated with high milk yield
作者:Zhao, Jianqing;Shi, Chenbo;Kamalibieke, Jiayidaer;Mu, Yuanpan;Zhu, Lu;Wang, Wei;Luo, Jun;Gong, Ping;Lv, Xuefeng
关键词:Dairy goat; Selective signal analysis; Genome-wide association study; Transcriptomic analyses; Milk yield
-
Smartphone-assisted electrochemiluminescence imaging test strips towards dual-signal visualized and sensitive monitoring of aflatoxin B1 in corn samples
作者:Chen, Miao-Miao;Jiang, Jun;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Chen, Miao-Miao;Jiang, Jun;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Tang, Xiaoqian;Zhang, Qi;Li, Peiwu;Chen, Miao-Miao;Zhang, Min-Ling;Song, Xiao;Zhang, Xiuhua;Chen, Miao-Miao;Song, Xiao
关键词:Electrochemiluminescence imaging; Test strips; Nanomaterials; Dual-signal outputs; Aflatoxin B1; Food safety
-
Detection and characterization of bovine hepacivirus in cattle and sheep from Hulunbuir, northeastern China
作者:Ma, Jingge;Liu, Ziyan;Liu, Ning;Wang, Zedong;Ma, Jingge;Wei, Feng;Wei, Zhiwei;Zheng, Xiangyu;Li, Liang;Liu, Ziyan;Wang, Wei
关键词:bovine hepacivirus (BovHepV); sheep; cattle; phylogenetic evolution; China
-
Effects of maternal Escherichia coli lipopolysaccharide exposure on offspring: insights from lncRNA analysis in laying hens
作者:Yan, Zhixun;Liu, Huagui;Chu, Qin;Liu, Lei;Liu, Lei;Yu, Ying;Wang, Wei;Adetula, Adeyinka Abiola
关键词:LPS maternal stimulation; Chicken; Offspring; Egg-laying rate; lncRNA
-
Comparative transcriptome profiling reveals the key genes and molecular mechanisms involved in rice under blast infection
作者:Li, Gang;Wang, Jian;Cheng, Baoshan;Wang, Di;Gao, Hao;Xu, Weijun;Wang, Wei;Gao, Qingsong;Zhang, Wenxia;Ji, Jianhui;Li, Bianhao;Zhang, Guoliang;Qi, Zhongqiang;Liu, Yongfeng
关键词:Rice; Blast; Transcriptome; Disease resistance; Hormones; Biochemical indicators
-
Ac/Ds-like Transposon Elements Inserted in ZmABCG2a Cause Male Sterility in Maize
作者:Wang, Le;Arshad, Saeed;Li, Taotao;Wei, Mengli;Jia, Haiyan;Ma, Zhengqiang;Yan, Yuanxin;Ren, Hong;Wang, Wei;Yan, Yuanxin
关键词:maize (
Zea mays ); seed production;ZmABCG2a ; mutantms*-N125 ; mutantms*-P884 -
Visible light photocatalytic degradation of pesticides and antibiotics by H3PO4-activated biochar combined with g-C3N4: Effects, mechanism, degradation pathway, and toxicity assessment
作者:Shi, Haojie;Wang, Wei;Mao, Liangang;Zhang, Lan;Zhu, Lizhen;Wu, Chi;Liu, Xingang
关键词:Biochar; Pesticide; Antibiotic; Photodegradation