Presence of a short repeat sequence in internal transcribed spacer (ITS) 1 of the rRNA gene of Sogatella furcifera (Hemiptera: Delphacidae) from geographically different populations in Asia

文献类型: 外文期刊

第一作者: Matsumoto, Yukiko

作者: Matsumoto, Yukiko;Sato, Yuki;Noda, Hiroaki;Fu, Qiang;Matsumura, Masaya

作者机构:

关键词: clones;genes;geographical distribution;insect pests;intergenic DNA;nucleotide sequences;plant pests;population genetics;populations;ribosomal RNA;insects;Laodelphax striatellus;Nilaparvata lugens;Sogatella furcifera;ACP Countries;animals;APEC countries;arthropods;ASEAN Countries;Asia;Auchenorrhyncha;Australasia;Commonwealth of Nations;Delphacidae;Developed Countries;Developing Countries;East Asia;eukaryotes;Fulgoroidea;Hemiptera;Hexapoda;Homoptera;Indochina;insects;invertebrates;Laodelphax;Melanesia;New Guinea;Nilaparvata;Oceania;OECD Countries;Pacific Islands;Sogatella;South East Asia.

期刊名称:APPLIED ENTOMOLOGY AND ZOOLOGY ( 影响因子:1.403; 五年影响因子:1.381 )

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年卷期:

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收录情况: SCI

摘要: Nucleotide sequences of the internal transcribed spacer 1 (ITS1)-5.8S-ITS2 region of the nuclear ribosomal RNA gene were determined in the white-backed planthopper (WBPH) Sogatella furcifera (Horvath) to detect molecular variation among regional populations in Asia. We analyzed 932 sequences from 172 individuals (4-9 clones per individual) of 33 populations collected in 1987-2008 from six countries, Japan, China, Taiwan, Vietnam, Philippines, and Papua New Guinea. WBPH showed intra-individual variation in ITS1, which is mainly attributable to the frequency (0-10) of the 66-bp repeat sequence in ITS1. Among the examined clones, the sequences of 5.8S were mostly identical and those of ITS2 were similar. A single planthopper had a maximum of 6 different variants in the number of ITS1 repeats, suggesting highly varied repeat numbers in individual planthoppers. The ITS1 with four repeats was the most frequently (64%) detected. Such a repeat was not observed in two other economically important planthopper species, Nilaparvata lugens (StAyenl) and Laodelphax striatellus (Fallen). The ITS nucleotide sequences in the WBPH populations in Asia were genetically close and some variations in the sequences were not related to regional populations, indicating that the nucleotide sequences of the ITS region are not useful for geographical discrimination of the WBPH. This closeness seems to be caused by long distance migration and genetic exchange among populations.

分类号: Q96

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