Phylogenetic study of Baylisascaris schroederi isolated from Qinling subspecies of giant panda in China based on combined nuclear 5.8S and the second internal transcribed spacer (ITS-2) ribosomal DNA sequences

文献类型： 外文期刊

第一作者： Zhao, Guang-Hui

作者： Zhao, Guang-Hui;Li, Hong-Mei;Cong, Mei-Mei;Hu, Bing;Gao, Man;Ren, Wan-Xin;Wang, Xing-Ye;Zhang, Shui-Ping;Lin, Qing;Yu, San-Ke;Zhao, Guang-Hui;Li, Hong-Mei;Zhu, Xing-Quan;Yu, San-Ke;Ryan, Una M.;Zhu, Xing-Quan

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关键词： Baylisascaris schroederi;Giant panda;Qinling subspecies;5.8S rDNA;ITS-2 rDNA;Ascarididae

期刊名称：PARASITOLOGY INTERNATIONAL （ 影响因子：2.23； 五年影响因子：2.281 ）

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收录情况： SCI

摘要： The nuclear ribosomal DNA (rDNA) region spanning 5.8S rDNA and the second internal transcribed spacer (ITS-2) of Baylisascaris schroederi isolated from the Qinling subspecies of giant panda in Shaanxi Province, China were amplified and sequenced. Sequence variations in the two rDNA regions within B. schroederi and among species in the family Ascarididae were examined. The lengths of B. schroederi 5.8S and ITS-2 rDNA sequences were 156 bp and 327 bp, respectively, and no nucleotide variation was foundin these two rDNA regions among the 20 B. schroederi samples examined, and these ITS-2 sequences were identical to that of B. schroederi isolated from giant panda in Sichuan province, China. The inter-species differences in 5.8S and ITS-2 rDNA sequencesamong members of the family Ascarididae were 0-1.3% and 0-17.7%, respectively. Phylogenetic relationships among species in the Ascarididae were re-constructed by Bayesian inference (Bayes), maximum parsimony (MP), and maximum likelihood (ML) analyses, based on combined sequences of 5.8S and ITS-2 rDNA. All B. schroederi samples clustered together and sistered to B. transfuga with high posterior probabilities/bootstrap values, which further confirmed that nematodes isolated from the Qinling subspecies of giant panda in Shaanxi Province, China represent B. schroederi. Because of the large number of ambiguously aligned sequence positions (difficulty of inferring homology by positions), ITS-2 sequence alone is likely unsuitable for phylogenetic analyses at the family level, but the combined 5.8S and ITS-2 rDNA sequences provide alternative genetic markers for the identification of B. schroederi and for phylogenetic analysis of parasites in the family Ascarididae.

分类号： Q18

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