文献类型： 外文期刊
第一作者： Chen, Yumei
作者： Chen, Yumei;Wan, Bo;Wang, Yinbiao;Zhang, Gaiping;Liu, Yunchao;Chang, Junbiao;Chang, Junbiao;Chen, Yumei;Zhang, Gaiping
作者机构：
期刊名称：BIOTECHNOLOGY LETTERS （ 影响因子：2.461； 五年影响因子：2.457 ）
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年卷期：
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收录情况： SCI
摘要： A non-immunized human single chain variable fragment (scFv) library containing 2.5 x 10(7) individual clones was constructed from antibody variable region genes of 200 non-immunized donors. ScFv gene repertories were generated by randomly combining rearranged variable regions of heavy chain (VH) and natural occurring light chain (VL) using overlapping extension PCR (OE-PCR). Five recombinant protein antigens from different species were successfully used to select specific binders. Phage ELISA showed that the recombinant phage particle could specifically bind to non-structural protein 1 of Avian influenza virus. This method can therefore efficiently generate a phage antibody library.
分类号： Q7
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