Effect of a DNA vaccine harboring two copies of inhibin alpha (1-32) fragments on immune response, hormone concentrations and reproductive performance in rats

文献类型： 外文期刊

第一作者： Wang, Shui-Lian

作者： Wang, Shui-Lian;Xue, Li-Qun;Han, Li;Ahmad, S.;Liang, Ai-Xin;Yang, Li-Guo;Ahmad, S.;Cao, Shao-Xian;Xing, Zhao-Fang;Feng, Jian-Zhong

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期刊名称：THERIOGENOLOGY （ 影响因子：2.74； 五年影响因子：2.93 ）

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收录情况： SCI

摘要： The objective was to investigate the effects of a novel DNA vaccine (pcISI) harboring two copies of inhibin alpha (1-32) fragments on immune response, hormone concentrations and reproductive performance in rats. Female Wistar rats (n = 18 per group) were immunized (twice, 4 wk apart) with 10, 50, or 100 mu g (T-1, T-2 and T-3, respectively), of the pcISI plasmid. At 4 wk after the second immunization, plasma antibody titers were higher (P < 0.05) in T-3 than in either T-1 or T-2 (0.341 +/- 0.123, 0.236 +/- 0.068, and 0.251 +/- 0.077, respectively, mean +/- SD). Concurrrently, plasma concentrations of FSH and estradiol were highest (P < 0.05) in T-3, and were higher (P < 0.05) in T-1 and T-2 than in control groups. For antibody-positive rats, there was a correlation (P < 0.01) between antibody titer and FSH concentrations after two pcISI immunizations. The number of mature follicles in the T-3 group (46.00 +/- 4.65) was higher (P < 0.05) than in two control groups (29.25 +/- 3.72 and 27.92 +/- 3.48), and also higher (P < 0.05) than in T-1 and T-2 (37.17 +/- 4.99 and 38.75 +/- 7.09). Antibody-positive rats had more mature ovarian follicles than negative rats (46.75 +/- 4.23 vs. 35.60 +/- 3.38, P < 0.05). Moreover, litter size and number of placentas were increased (P < 0.05) in the pcISI immunization groups, except for the T-1 group, compared to the control groups. In conclusion, the pcISI DNA vaccine successfully induced a humoral immune response, improved reproductive hormone concentrations, stimulated follicular development, and increased number of placentas and litter size. Furthermore, 100 mu g yielded the best immune response. (c) 2012 Elsevier Inc. All rights reserved.

分类号： Q95

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