A loop-mediated isothermal amplification method for rapid detection of the multidrug-resistance gene cfr

文献类型： 外文期刊

第一作者： Qi, Jing

作者： Qi, Jing;Du, Yijun;Zhu, Rongsheng;Zhu, Xiaoling;Bai, Hua;Hu, Ming;Luo, Yanbo;Hu, Xinxin;Liu, Yuqing;Wu, Congming;Shen, Jianzhong

作者机构：

关键词： Loop-mediated isothermal amplification (LAMP);Rapid detection;Sensitivity;The multidrug-resistance gene cfr

期刊名称：GENE （ 影响因子：3.688； 五年影响因子：3.329 ）

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年卷期：

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收录情况： SCI

摘要： We developed and evaluated the specificity and sensitivity of a loop-mediated isothermal amplification (LAMP) method for rapid detection of the multidrug-resistance gene . cfr. A pair of outer primers and a pair of inner primers and one loop primer were specially designed for recognizing seven distinct sequences on the target . cfr gene. The amplification reaction was performed within only 35. min under isothermal conditions at 63. °C in a regular water bath with visual measurement. The LAMP assay showed higher sensitivity than the conventional PCR, with a detection limit of 1. pg per tube of chicken . Staphylococcus sciuri genomic DNA. The detection rate of . cfr gene for 50 Staphylococcus clinical strains by LAMP assays was 16% and appeared 100% consistence with the result by PCR method. The LAMP method reported here demonstrated a potential and valuable means for detection of the multidrug-resistance gene . cfr: easy, rapid, visual, specific, accurate and sensitive, especially useful for on-the-spot investigation.

分类号： R394

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