Identification of three new type-specific antigen epitopes in the capsid protein of porcine circovirus type 1

文献类型： 外文期刊

第一作者： Huang, Liping

作者： Huang, Liping;Lu, Yuehua;Wei, Yanwu;Guo, Longjun;Liu, Changming

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期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.574； 五年影响因子：2.466 ）

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收录情况： SCI

摘要： Porcine circovirus type 1 (PCV1) has been identified as a contaminant of porcine kidney cell line (PK-15). Serological evidence and genetic studies have suggested that PCV1 is widespread in domestic pigs. In this study, monoclonal antibodies (mAbs) and polyclonal antibodies (pAbs) were generated against a recombinant PCV1 Cap protein (PCV1-Cap), which was expressed using the baculovirus system. PEPSCAN analysis was used to identify epitopes on the PCV1-Cap with mAbs and pAbs. Three linear B-cell epitopes, including residues 85GGTNPLP 91, 162FTPKPELDKTIDWFHPNNK 180 and 219YVQFREFILKDPLNK 233, specific for PCV1-Cap, were finely defined. These results will facilitate future investigations into antigenic differences and differential diagnosis between PCV1 and PCV2.

分类号： R37

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