Enhancement of the immunogenicity of an alphavirus replicon-based DNA vaccine against classical swine fever by electroporation and coinjection with a plasmid expressing porcine interleukin 2

文献类型： 外文期刊

第一作者： Tian, Da-Yong

作者： Tian, Da-Yong;Sun, Yuan;Meng, Qi-Lin;Wang, Nan;Han, Wen;Li, Su;Li, Yong-Feng;Li, Dan;Ling, Li-Jun;Liao, Ya-Jin;Qiu, Hua-Ji;Wai, Sing Fai;Lee, Fuk Ki;Suen, Kar Man

作者机构：

关键词： Classical swine fever;Alphavirus replicon;Electroporation;Interleukin 2

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： Alphavirus replicon-based DNA vaccines have emerged as a promising approach to generation of antigen-specific immune responses. However, due to their low immunogenicity, there is a need for other approaches to enhance the vaccine potency. In this study, electroporation (EP) and a plasmid expressing porcine interleukin 2 (IL-2) were used to improve the immunogenicity of an alphavirus replicon-based DNA vaccine pSFV1CS-E2 against classical swine fever (CSF). Pigs were immunized with pSFV1CS-E2 alone or together with IL-2 by EP or by simple intramuscular injection. The results showed that EP combined with IL-2 resulted in marked enhancement of E2-specific antibody responses. Moreover, CSFV-specific lymphocyte proliferation, IFN-gamma and IL-4 responses were increased significantly in the pSFV1CS-E2+1L-2/EP group. Pigs immunized with pSFV1CS-E2 plus IL-2 by EP were completely protected from lethal challenge, which is comparable to the sterilizing immunity and full protection offered by the live attenuated vaccine C-strain and in contrast with the incomplete protection conferred by pSFV1CS-E2 without or with IL-2 or EP alone, as demonstrated by the presence of pathological changes or/and viral loads. We conclude that EP in combination with IL-2 can significantly improve the immunogenicity of the plasmid DNA vaccine. (C) 2012 Elsevier Ltd. All rights reserved.

分类号： R392

  • 相关文献

[1]A novel alphavirus replicon-vectored vaccine delivered by adenovirus induces sterile immunity against classical swine fever. Sun, Yuan,Li, Hong-Yu,Tian, Da-Yong,Han, Qiu-Ying,Zhang, Xin,Li, Na,Qiu, Hua-Ji. 2011

[2]Enhanced immunogenicity induced by an alphavirus replicon-based pseudotyped baculovirus vaccine against porcine reproductive and respiratory syndrome virus. Wu, Qunfeng,Xu, Fengqin,Fang, Liurong,Xu, Jinfang,Li, Bin,Jiang, Yunbo,Chen, Huanchun,Xiao, Shaobo,Li, Bin. 2013

[3]Suppression and restoration of primordial germ cell marker gene expression in channel catfish, Ictalurus punctatus, using knockdown constructs regulated by copper transport protein gene promoters: Potential for reversible transgenic sterilization. Su, Baofeng,Shang, Mei,Peatman, Eric,Perera, Dayan A.,Cheng, Qi,Li, Chao,Weng, Chia-Chen,Li, Ping,Liu, Zhanjiang,Dunham, Rex A.,Su, Baofeng,Shang, Mei,Grewe, Peter M.,Patil, Jawahar G.,Patil, Jawahar G.,Perera, Dayan A.,Li, Chao,Li, Ping.

[4]Enhanced immunity against classical swine fever in pigs induced by prime-boost immunization using an alphavirus replicon-vectored DNA vaccine and a recombinant adenovirus. Sun, Yuan,Li, Na,Li, Hong-Yu,Li, Miao,Qiu, Hua-Ji. 2010

[5]A high efficiency gene disruption strategy using a positive-negative split selection marker and electroporation for Fusarium oxysporum. Liang, Liqin,Ling, Jian,Luo, Zhongqin,Xie, Bingyan,Liang, Liqin,Li, Jianqiang,Liang, Liqin,Cheng, Lin,Bai, Miao.

[6]Classical swine fever in China: A minireview. Luo, Yuzi,Li, Su,Sun, Yuan,Qiu, Hua-Ji.

[7]Piglets with maternally derived antibodies from sows immunized with rAdV-SFV-E2 were completely protected against lethal CSFV challenge. Xia, Shui-Li,Du, Mingliang,Lei, Jian-Lin,Liu, Yan,Wang, Yimin,Ji, Shengwei,Xiang, Guang-Tao,Li, Lian-Feng,Cong, Xin,Luo, Yuzi,Shao, Lina,Qiu, Hua-Ji,Sun, Yuan.

[8]Comprehensive evaluation of the adenovirus/alphavirus-replicon chimeric vector-based vaccine rAdV-SFV-E2 against classical swine fever. Qiu, Hua-Ji. 2013

[9]Efficacy of the marker vaccine rAdV-SFV-E2 against classical swine fever in the presence of maternally derived antibodies to rAdV-SFV-E2 or C-strain. Xia, Shui-Li,Xiang, Guang-Tao,Lei, Jian-Lin,Du, Mingliang,Wang, Yimin,Zhou, Mo,Liu, Yan,Ji, Shengwei,Wang, Ya-Lin,Luo, Yuzi,Sun, Yuan,Qiu, Hua-Ji.

[10]Safety and immunogenicity of a gE/gI/TK gene-deleted pseudorabies virus variant expressing the E2 protein of classical swine fever virus in pigs. Lei, Jian-Lin,Xia, Shui-Li,Wang, Yimin,Du, Mingliang,Xiang, Guang-Tao,Cong, Xin,Luo, Yuzi,Li, Lian-Feng,Zhang, Lingkai,Yu, Jiahui,Qiu, Hua-Ji,Sun, Yuan,Lei, Jian-Lin,Hu, Yonghao.

作者其他论文 更多>>

微信小程序