Extraction of High-Quality RNA from Rubber Tree Leaves

文献类型： 外文期刊

第一作者： Luo, Ming-Wu

作者： Luo, Ming-Wu;Deng, Liu-Hong;Zhang, Chun-Fa;Zeng, Hui-Cai

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关键词： rubber-tree leaves;RNA extraction;cetyltrimethylammonium bromide (CTAB);TRIzol;rapid amplification of cDNA ends (RACE)

期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：2.043； 五年影响因子：2.017 ）

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收录情况： SCI

摘要： A specific technique capable of producing high-quality RNA for rapid amplification of cDNA ends (RACE) was established for challenging tissues: leaves of the rubber tree. Total RNA was extracted by cetyltrimethylammonium bromide (CTAB)-LiCl combined with TRIzol reagent. The isolated RNA was highly intact. With RNA as template, full-length cDNA was obtained (NCBI, AY461413) by RACE.

分类号： Q

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