Development of method for preparing high molecular weight DNA from chrysanthemum

文献类型： 外文期刊

第一作者： Guo, Xinwei

作者： Guo, Xinwei;Zhang, Xiuhai;Wu, Zhongyi;Luo, Chang;Cheng, Xi;Huang, Conglin

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关键词： Chrysanthemum lavandulifolium;High molecular weight DNA;Phenolic compound

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：3.463； 五年影响因子：3.672 ）

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收录情况： SCI

摘要： We developed an improved method for preparing high molecular weight DNA (HMW-DNA) from leaves of Chrysanthemum lavandulifolium that has high content of phenolic compounds. Compared with three previous methods for preparing HMW-DNA, only with the improved method a considerable yield of HMW-DNA more than 2 Mb was achieved. The improved method utilizes a modified buffer system including 1% (v/v) beta-mercaptoethanol, 4 mM spermidine and 6% (w/v) polyvinylpyrrolidone (PVP)-40 and a centrifugal speed of 1600 x g to extract and suspend HMW-DNA. With the improved method, phenolic compounds were successfully removed and antioxidants protected HMW-DNA from oxidation and degradation. No difference on the yield of DNA was observed with different lysis times. The HMW-DNA can be partially digested to 23-80 kb in size, and is suitable for artificial chromosome library construction

分类号： S6

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