Chloroplast subspecies-specific SNP detection and its maternal inheritance in Brassica oleracea L. by using a dCAPS marker

文献类型: 外文期刊

第一作者: Zhang, Yangyong

作者: Zhang, Yangyong;Fang, Zhiyuan;Wang, Qingbiao;Liu, Yumei;Yang, Limei;Zhuang, Mu;Sun, Peitian

作者机构:

关键词: B. oleracea L.;chloroplast;maternal inheritance;subspecies-specific SNP

期刊名称:JOURNAL OF HEREDITY ( 影响因子:2.645; 五年影响因子:3.069 )

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收录情况: SCI

摘要: Chloroplast simple sequence repeats amplicons in 5 subspecies of Brassica oleracea were sequenced, and one chloroplast SNP was detected in amplicon ACP43. Through the introduction of an RsaI recognition site by adding one mismatch in the forward primer, combined with the increased primer length and raised annealing temperature, the dCAPS (derived cleaved amplified polymorphic sequences) marker ACP43-93 RsaI was successfully developed. By using the dCAPS marker, the subspecies-specific SNP was assayed in 206 materials representing the wide distribution of B. oleracea. This is the first report of chloroplast DNA (cpDNA) variation in cultivated subspecies of B. oleracea, which showed that chloroplast diversity existed at the intersubspecies level. Unlike other subspecies, most of the broccoli and all of the cauliflower materials sharing the same haplotype showed closer relationships in cpDNA level. Furthermore, the dCAPS haplotype of the offspring from 7 male sterile backcross populations was the same as the female parents, indicating maternal inheritance.

分类号: R394

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