Isolation of laccase gene from Bacillus subtilis and analysis of its physicochemical properties
文献类型: 外文期刊
第一作者: Yang, Sui-Shan
作者: Yang, Sui-Shan;Liu, Zhen-Wang;Yi, Xiao-Ping;Zhang, Ai-Lian;Zhang, Ze-Hua;Shen, Jin-Cheng;Yin, Hui-Xiang;Chen, Li-Ping;Zhang, Tian-Yuan;Luo, Jin-Xian;Zhang, Tian-Yuan;Luo, Jin-Xian
作者机构:
关键词: Enzyme activity;Expression;Gene cloning;Laccase;Physicochemical property
期刊名称:GENE ( 影响因子:3.688; 五年影响因子:3.329 )
ISSN:
年卷期:
页码:
收录情况: SCI
摘要: The present study reports the cloning and sequencing of lac2 from Bacillus subtilis. The gene is composed of 1542 bp and encodes a 514-amino acid protein. The gene has 86% homology with a published laccase with GeneID 936023. The lac2 gene was deposited in GenBank as a new nucleotide sequence. This new sequence was cloned into the multiple cloning site of pPIC9K to generate pPIC9K-lac2, which was then transformed into Pichia pastoris GS115 via electroporation. The recombinant GS115 (pPIC9K- lac2) was grown initially in BMGY medium and transferred to BMMY to induce gene expression for 48 h. The recombinant Lac2 protein shows laccase activity with α-naphthol and guaiacol as substrates. The optimal pH is between 3.2 and 4.7, and the optimal temperature is 25. °C for enzyme reaction.
分类号: R394
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