Identification and genomic analysis of two duck-origin Tembusu virus strains in southern China

文献类型: 外文期刊

第一作者: Li, Linlin

作者: Li, Linlin;An, Hejia;Sun, Minhua;Dong, Jiawen;Yuan, Jianfeng;Hu, Qilin;An, Hejia

作者机构:

关键词: Duck;Duck-origin Tembusu virus;Egg drop;Phylogenetic analysis

期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )

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年卷期:

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收录情况: SCI

摘要: Egg-laying duck flocks in Guangdong province, southern China, have been suffering a widely spreading infectious disease with abrupt egg drops and death since the winter of 2010. However, the causative pathogen was not known. We obtained two independent virus isolates named FS and JM from the diseased layer duck flocks and identified them as duck-origin Tembusu virus by PCR detection, sequencing the entire length of the open reading frames (ORFs). The two isolates FS and JM shared high sequence similarity to the isolates of duckorigin Tembusu virus that was first emerged in eastern China in April 2010. Blast analysis shows that the whole ORF sequences of FS and JM have the highest similarity ([99 %) to BYD-1(the first reported duck-origin Tembusu virus) and JS804 (the first reported goose-origin Tembusu virus), indicating that the full-length genomes were highly conserved in waterfowl-origin Tembusu viruses. The present study suggests that duck-origin Tembusu viruses have spread fast from eastern China to southern China, causing widely spreading infections. The high conservation of duck-origin Tembusu virus strains provides the genomic basis for choosing the strains for vaccine preparation for better protection against this new virus infection.

分类号: R37

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