Isolation of five bioactive components from eupatorium adenophorum Spreng using stepwise elution by high-speed countercurrent chromatography

文献类型： 外文期刊

第一作者： Wei, Yun

作者： Wei, Yun;Zhang, Kai;Zhang, Guoliang;Ito, Yoichiro

作者机构：

关键词： 4′-methyl quercetagetin 7-O-(6″-O-E-caffeoylglucopyranoside);eupalitin;eupalitin 3-O-β-D-galactopyranoside;Eupatorium adenophorum Spreng;quercetagetin 7-O-(6″-O-acetyl-β-D-glucopyranoside);stepwise high-speed countercurrent chromatography

期刊名称：JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES （ 影响因子：1.312； 五年影响因子：1.145 ）

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收录情况： SCI

摘要： Semi-preparative high-speed countercurrent chromatography (HSCCC) was successfully performed for isolation and purification of caffeic acid and 4 bioactive flavonoids from Eupatorium adenophorum Spreng using stepwise elution with a pair of two-phase solvent systems composed of ethyl acetate-methanol- water at the volume ratios of 10:1:10 and 5:1:5 (v/v). From 378.5mg of crude extract 24.1mg of caffeic acid, 6.7mg of 4′-methyl quercetagetin 7-O-(6″-O-E-caffeoylglucopyranoside), 6.5mg of quercetagetin 7-O-(6″-O-acetyl - D-glucopyranoside), 31.8mg of eupalitin 3-O-β-D-galactopyranoside, and 36.7mg of eupalitin were obtained with purities of 96.0%, 91.2%, 82.3%, 95.1%, and 85.6%, respectively. The structures of the separated compounds were identified by EI-MS, FAB-MS, 1HNMR, and 13CNMR.

分类号： O661

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[3]A new Sequiterpenoid from Eupatorium adenophorum Spreng. Yang Jie,Cao Ao-Cheng,Liu Yu-Mei,An Yu,Shi Jian-Gong. 2006

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